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| 以黄芩苷溶出量探讨黄芩与半夏配伍药性变化 | |
| 引用本文： | 李平,杨应勇,李江,肖书伟.以黄芩苷溶出量探讨黄芩与半夏配伍药性变化[J].中国实验方剂学杂志,2011,17(17):119-122. |
| 作者姓名： | 李平 杨应勇 李江 肖书伟 |
| 作者单位： | 1. 贵阳中医学院第二附属医院,贵阳,550002 2. 贵州神奇药业集团苗药工程中心,贵阳,550002 3. 贵阳中医学院,贵阳,550002 |
| 基金项目： | 吉林省科委重点项目(20106041) |
| 摘 要： | 目的:采用3种方法对五味子进行蛋白质提取,寻找一种简单有效的五味子蛋白提取方法。方法:利用SDS-聚丙烯酰胺凝胶电泳法对蛋白提取物进行分析,同时依据Lowry法对五味子总蛋白含量进行测定,HPLC测定五味子蛋白提取物中氨基酸的含量。结果:3种方法提取总蛋白质量分数分别为58.44%,19.21%,26.18%,其所含氨基酸质量分数分别为57.46%,15.41%,23.14%。结论:采用脱脂硫酸铵沉降法提取所得总蛋白及其所含氨基酸含量较高、纯度较合理,方法较可行。 |
| 关 键 词： | 五味子 蛋白 氨基酸 含量测定 |
| 收稿时间： | 2011/3/23 0:00:00 |
Medicine Property Variation in Different Proportional Combinations of Scutellariae Radix and Prepared Pinellia Tuber through Determination Leaching Quantity of Baicalin by HPLC |
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| LI Ping,YANG Ying-yong,LI Jiang and XIAO Shu-wei.Medicine Property Variation in Different Proportional Combinations of Scutellariae Radix and Prepared Pinellia Tuber through Determination Leaching Quantity of Baicalin by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2011,17(17):119-122. | |
| Authors: | LI Ping YANG Ying-yong LI Jiang and XIAO Shu-wei |
| Institution: | Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;Department of Experimental Center of Miao National Medicine, Guizhou Shenqi Pharmacy Co.Ltd., Guiyang 550002, China;Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;Department of Experimental Center of Miao National Medicine, Guizhou Shenqi Pharmacy Co.Ltd., Guiyang 550002, China |
| Abstract: | |
| Keywords: | Scutellariae Radix Prepared Pinellia tuber baicalin compatibility |
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