用细胞膜色谱法和HPLC-TOF/MS研究附子中的有效成分

目的通过细胞膜色谱技术和高效液相-飞行时间质谱（HPLC-TOF／MS）技术相结合，初步筛选和鉴定附子中的有效成分。方法初步筛选采用自制的大鼠心肌细胞膜色谱柱，流动相为10mmol／L磷酸盐缓冲溶液，流速为0．2ml／min。色谱分离采用SHISEIDO CAPCELL PAKC18柱，流动相为0．1％甲酸水溶液和乙腈，梯度洗脱，流速为1．0ml／min。质谱鉴定采用TOF／MS，正离子模式扫描。结果确定了附子中8个可能的有效成分。结论通过细胞膜色谱技术和HPLC-TOF／MS技术相结合，为从天然产物中快速寻找新的活性成分提供了途径。

Study on effective components from roots of Aconitum carmichaeli through cell membrane chromatography and HPLC-TOF/MS method

Objective To screen the effective components from roots of Aconitum carmichaeli by a cell membrane chromatography (CMC) combining with a HPLC-TOF/MS method. Methods Screening was conducted on a rat cardiac muscle CMC column, and mobile phase was 10 mmol/L phosphate buffer with a flow rate of 0.2 ml/min. Separation was conducted on a SHISEIDO CAPCELL PAK C₁₈ column. The mobile phase consisted of 0.1% formic acid and acetonitrile using gradient elution with a flow rate of 1.0 ml/min. Identification was conducted by using TOF/MS in positive ion mode. Results Eight potential effective constituents were determined. Conclusion The combination of CMC and HPLC-TOF/MS method had provided a new way to discover active components targeting membrane receptors from natural products.