舒郁胶囊对抑郁情绪模型大鼠海马和下丘脑内5-羟色胺3B受体分布与表达的影响

目的:检测抑郁情绪模型大鼠海马、下丘脑内5-羟色胺3B受体(5-HT3BR)蛋白分布、表达的变化,初步探讨舒郁胶囊对抑郁情绪的干预机制。方法:筛选大鼠进入实验,分为正常组、模型组、舒郁胶囊组(0.4 g.kg-1)、氟西汀组(0.002 g.kg-1),采用慢性温和性刺激法(chronic mild stress,CMS)制备抑郁情绪大鼠模型,以造模前后体重及糖水偏好值评价模型,采用免疫荧光技术检测大鼠海马和下丘脑内5-HT3B R的表达分布,以平均荧光强度(MFI)为指标对各组大鼠海马和下丘脑内5-HT3BR进行定量。结果:与正常组大鼠相比,模型组大鼠体重显著减轻(P<0.01),糖水摄入量也显著减少(P<0.01),5-HT3BR蛋白在海马和下丘脑中的表达水平显著升高(P<0.01);与模型组相比,舒郁胶囊组和氟西汀组大鼠体重增长显著(P<0.01),糖水摄入量显著增加(P<0.01),5-HT3BR蛋白在海马和下丘脑中的表达水平明显降低(P<0.01),但两组之间没有显著性差异。结论:5-HT3BR表达水平的上调可能与抑郁情绪的产生关系密切;舒郁胶囊可对抑郁情绪模型大鼠海马、下丘脑5-HT3BR表达水平的变化起到纠正作用。

Effect of Shuyu Capsule on Location and Expression of 5-hydroxy Tryptamine3B Receptor in Hippocampus and Hypothalamus Regions in Rats with Depression Emotion

Objective: To detect the changes in protein location and expression level of 5-hydroxy tryptamine_3B receptor in related regions of hippocampus and hypothalamus in rats with depression emotion (DE),to explore the intervention mechanism of Shuyu capsule for treating DE preliminarily. Method: The Wistar rats were divided into normal group,model group,Shuyu capsule group(0.4 g ·kg^-1) and fluoxetine group(0.002 g ·kg^-1). Then we used the chronic mild stress(CMS)to establish the DE model in rats, and then the rats were evaluated by sucrose intake and the body weight from beginning to the end for the model. We adopted the immune fluorescence to detect the protein distribution and expression levels of 5-HT_3B receptor in related region of hippocampus and hypothalamus,and to calculate the content of the receptors in the regions by the mean fluorescence intensity(MFI) as the index. Result: Compared with the normal group,the body weight of rats in DE model group lessened significantly (P<0.01),the sucrose intake of preference also decreased obviously (P<0.01),the expression of the receptor of 5-HT_3B had a significant increase in hippocampus and hypothalamus (P<0.01). Compared with the model group,in the Shuyu capsule group and fluoxetine group,the body weight of rats in DE model group increased significantly (P<0.01),the sucrose intake of preference also increased obviously (P<0.01),the expression of the receptor of 5-HT_3B was increased markedly in hippocampus and hypothalamus (P<0.01),however,there was no statistical differences in the two treatment groups. Conclusion: The high expression of 5-HT_3B receptor may have close relationship with DE,and Shuyu Capsule can correct the high expression of the 5-HT_3B receptor protein in the hippocampus and hypothalamus of DE model in rats.