黄芪注射液对H22荷瘤小鼠瘤组织Bax 及Bcl-2蛋白表达的影响

目的:研究黄芪注射液对H22荷瘤小鼠瘤组织Bax及Bcl-2蛋白表达的影响。方法:建立H22荷瘤小鼠模型进行体内抗肿瘤试验,将50只荷瘤小鼠随机分为空白对照组(模型组)、环磷酰胺组(CTX组)、黄芪注射液(高、中、低剂量)组,连续ip给药10 d,观察肿瘤生长情况以及小鼠一般情况,绘制肿瘤生长曲线。10 d后处死小鼠,剥取瘤组织称重,计算抑瘤率;采用流式细胞术测定药物作用后肿瘤细胞凋亡率;免疫组化方法检测肿瘤组织Bax及Bcl-2的蛋白表达。结果:小鼠移植瘤H22试验结果显示,在128,,4 g.kg-1的剂量下黄芪注射液的抑瘤率分别为52.36%,33.68%,17.28%。流式细胞术及免疫组化结果表明黄芪注射液各剂量组均可明显增加肿瘤细胞凋亡率;增强Bax蛋白表达,降低Bcl-2蛋白表达,且Bax/Bcl-2明显增高(P<0.05)。结论:黄芪注射液通过促进Bax蛋白表达、抑制Bcl-2蛋白表达来诱导H22肿瘤细胞凋亡,进而发挥抑瘤效应。

Effects of Astragalus Injection on the Protein Expression of Bax and Bcl-2 in H22 Tumor Cells Bearing Mice

Objective: To investigate the effects of Astragalus injection on the protein expression of Bax and Bcl-2 in H22 tumor cells bearing mice. Method: Hepatic cancer H22 model in mice was performed to study anti-hepatocarcinoma activity of Astragalus injection in vivo. The growth curve and inhibitory rate of tumor growth were measured. Cell apoptosis of each group were measured by flow cytometry (FCM). Protein expression of Bax and Bcl-2 were analyzed by immunohistochemistry (IHC). Result: Under the treatment of 12,8,4 g ·kg^-1of Astragalus injection,the inhibitory rates of H22 transplanted tumor in mice were 52.36%,33.68%, 17.28%,respectively. FCM and IHC showed the cell apoptosis rate and protein expression of Bax and Bax/Bcl-2 ratio of H22 transplanted tumor in Astragalus injection were significantly higher than that of in the control group (P<0.05). The protein expression of Bcl-2 was significantly lower than that in control group (P<0.05). Conclusion: Astragalus injection has significant anti-tumor effects in vivo for inducing apoptosis of H22 tumor cells by promoting protein expression of Bax,decreasing protein expression of Bcl-2 gene,and marked increasing the Bax/Bcl-2 ratio.