大孔吸附树脂分离纯化芫花黄酮苷元工艺研究

目的:研究考察大孔吸附树脂用于芫花中2种主要黄酮苷元-芹菜素和芫花素的分离纯化效果。方法:采用静态和动态吸附法,利用高效液相对芹菜素和芫花素进行定量分析,筛选最佳分离纯化工艺。结果:最佳工艺条件为AB-8树脂,上样液的质量浓度0.1 g.mL-1,吸附流速2 BV.h-1,上样体积5 BV。再以9 BV 95%乙醇洗脱,洗脱流速2 BV.h-1,体积9 BV。经树脂处理后2种黄酮苷元纯度均达到20%以上。结论:大孔树脂对芫花中芹菜素和芫花素具有较好分离纯化效果,优选工艺操作简单、稳定可行。`

Studies on Separation and Purification of Flavonoid Aglycone from Flos Genkwa by Macroporous Adsorption Resin

Objective: To investigate the purification method of two main flavonoid aglycones (apigenin and genkwanin) from Flos Genkwa by macroporous resin. Method: HPLC was used to determine the content of apigenin and genkwanin, and the purification conditions were selected by static and dynamic adsorption. Result: AB-8 resin was selected because it gave the most effective of separation. The optimum process for the purification was as follows: the concentration of sample solution was 0.1 g ·mL^-1, the dosage was 5 BV, adsorption flow rate was 2 BV ·h^-1, and eluted with 95% ethanol by 9 BV, elution flow rate was 2 BV ·h^-1. The purity of two flavonoid aglycones was more than 20% after processing. Conclusion: Apigenin and genkwanin were purified effectively with macroporous resin. The optimum process is simple,stable and feasible.