活血化瘀方清除DPPH自由基活性量效关系

目的:对活血化瘀代表性方剂进行清除2,2-二苯基-1-苦肼基(DPPH)自由基活性评价。方法:采用DPPH自由基清除率法测定活血化瘀代表方血府逐瘀汤(XF)、少腹逐瘀汤(SF)、膈下逐瘀汤(GX)、身痛逐瘀汤(ST)、通窍活血汤(TQ),每个组方分别取全方、醇沉沉淀和醇沉上清液3个不同样品测清除DPPH自由基活性。结果:活血化瘀方各样品对DPPH自由基都有明显的清除作用,与空白比较有显著性差异(P<0.01),且有明显的量效关系,按生药量比较,5个全方较各分离部位效果好,全方清除自由基活性顺序为:GX>TQ>SF>ST>XF。其IC50按生药量计依次为0.93,1.40,1.50,2.31,2.32 g.L-1。结论:采用酶联免疫检测仪检测活血化瘀方对DPPH自由基的清除作用,方法简便,重复性好,适合高通量筛选中药及方剂的清除自由基活性。

Dose-response Relationship of Free Radical Scavenging Activity by Blood-activating and Stasis-removing Formulae

Objective: To study the effects of the free radical scavenging activity of these formulae for blood-activating and stasis-removing such as Xuefu Zhuyu Decoction(XF), Shaofu Zhuyu Decoction(SF), Gexia Zhuyu Decoction(GX), Shentong Zhuyu Decoction(ST)and Tongqiao Huoxue Decoction(TQ). Method: Free radical scavenging activity was determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH)method. Result: The result showed that all these formulae had significant effects on scavenging DPPH radical(P<0.01), in a dose-dependent manner.. The free radical scavenging capacity on DPPH were in the following descending order:GX>TQ>SF>ST>XF. The scavenging ability in total formula was better than in ethanol precipitant and supernatant. Conclusion: Validation studies proved that the enzyme-linked immunosorbent assay for DPPH had good reproducibility and feasibility. The method could be used as high throughput screening for Chinese herbs and traditional chinese medicine formulae.