膈下逐瘀汤抗大鼠免疫性肝纤维化的研究

目的:观察膈下逐瘀汤逆转大鼠肝纤维化的疗效和对肝损伤的保护作用。方法:大鼠随机分为空白对照组、膈下逐瘀汤组、猪血清组、猪血清+膈下逐瘀汤组。猪血清0.5 mL/只(2次/周)ip诱导大鼠免疫性肝纤维化模型,在制备模型的同时膈下逐瘀汤(含生药7.37 g.kg-1)ig干预,共12周。实验结束后处死大鼠,HE染色分析肝组织胶原纤维含量,ELISA法分析MMP胶原酶活性,分光光度法分析天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)活性。结果:与模型组比较,模型+膈下逐瘀汤组大鼠肝纤维化程度显著改善(U=2.57,P<0.05),AST和ALT活性显著降低(P<0.05),MMP胶原酶活性显著降低(P<0.05)。结论:膈下逐瘀汤对猪血清诱导的免疫性肝纤维化大鼠肝纤维化程度和肝损伤具有明显改善作用。

Effects and Mechanisms of Gexia Zhuyu Decoction on Immunological Hepatic Fibrosis in Experimental Rats

Objective: To investigate the mechanisms underlying the prevention effect of Gexia Zhuyu decoction against porcine serum-induced liver fibrosis in rats. Method : Sixty male Sprague-Dawley rats were randomly divided into four groups as follows: blank control group, Gexia Zhuyu decoction group, Porcine serum group, Porcine serum plus Gexia Zhuyu decoction group. The fibrosis model of rats was achieved by intraperitoneal injection of porcine serum twice weekly for 12 weeks, and simultaneously Gexia Zhuyu decoction administered daily by gavage for 12 weeks. All rats were sacrificed at the end of experiment, and their blood and liver were collected for analysis. The liver histopathology was examined by HE staining. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected by biochemicalanalysis. MMP collagenase activity in the liver were detected by enzyme linked immunosorbent assay. Result: Compared with the porcine serum group, the histopathology of rats given Gexia Zhuyu decoction improved significantly. ALT and AST in serum and MMP collagenase activity in liver were significantly decreased in porcine serum plus Gexia Zhuyu group (P < 0. 05). Conclusion : Gexia Zhuyu decoction may exert anti-hepatic fibrosis effect in rats via inhibition of hepatic injury and MMP collagenase activity.