乳香没药对细胞色素P450活性的影响

目的:考察乳香没药对细胞色素P450活性的影响。方法:56只小鼠分为对照组和乳香+没药3.15,2.10,1.05 g.kg-1剂量组,连续ig给药7 d;48只小鼠分为对照组和乳香、没药、乳香+没药组,ig给药3.15 g.kg-11次。所有小鼠末次药后30 min ip给予戊巴比妥钠,观察乳香没药对P450的影响。40只大鼠分为对照组、乳香组、没药组、乳香+没药组,以3.00 g.kg-1连续ig给药14 d,取肝脏制备各组动物肝微粒体,并用cocktail探针法考察乳香没药对大鼠CYP1A2,CYP2E1,CYP3A4的影响。结果:乳香+没药3.15,2.10,1.05 g.kg-1剂量连续给药7 d均可使戊巴比妥钠诱导的小鼠睡眠率降低(P<0.05,P<0.01),而单次给药3.15g.kg-1有使戊巴比妥钠诱导的小鼠睡眠率升高的趋势。乳香、没药及乳香+没药混合物组大鼠肝微粒体体外对非那西丁、氯唑沙宗代谢率显著高于对照组,而氨苯砜无明显差异。结论:乳香、没药连续用药均使CYP1A2,CYP2E1活性增强,而对CYP3A4则无显著影响。

Influence of Olibanum and Myrrha on Activity of Cytochrome P450

Objective: To investigate the influence of Olibanum and Myrrha on the activity of cytochrome P450 and its isoforms. Method: 56 mice were divided into control group and 3 groups treated with Olibanum+ Myrrha(3.15,2.10,1.05 g·kg^-1) for 7 days. And other 48 mice were divided into control group and 3 groups that were intragastrically administrated once with Olibanum+Myrrha, Olibanum, Myrrha(3.15 g·kg^-1). 30 min after last administration, mice were intraperitoneal injected with pentobarbital sodium, to observe the influence of Olibanum and Myrrha on P450. Forty rats were divided into four groups by random. 3 groups were intragastrically treated with Olibanum+ Myrrha, Olibanum, Myrrha(3.00 g·kg^-1) once a day for 14 days and the control group with water. Liver microsomes of rats was prepared to investigate the influence on CYP1A2, CYP2E1 and CYP3A4 of Olibanum and Myrrha by three-drug cocktail approach in vitro. Result: The number of sleeping mice was significantly decreased of groups which were treated with Olibanum and Myrrha(3.15,2.10,1.05 g·kg^-1) for 7 days, and yet the number was increased with once administration. The phenacetin and chlorzoxazone metabolic rates of Olibanum and Myrrha and its mixture groups were significantly higher than the control group in liver microsomes, and there was significantly different on dapsone. Conclusion: The activity of CYP1A2 and CYP2E1 was increased with the continuous use of Olibanum and Myrrha, and CYP3A4 was not significantly affected.