荆防败毒散加减对急性酒精中毒小鼠的解酒作用

目的:观察荆防败毒散加减对急性酒精中毒小鼠的影响。方法:小鼠随机分为5组,正常组,模型组,荆防败毒散加减高、中、低剂量组(按生药量计为32.4,16.2,8.1 g.kg-1,ig),除正常组外,采用56°红星二锅头酒14 mL.kg-1 ig建立急性酒精中毒小鼠模型,各组均1次性ig给药,预防性给药灌酒前30 min给药,观察对小鼠醉酒潜伏期、醉酒率的影响,治疗性给药灌酒16 mL.kg-1后30 min给药,观察对小鼠醒酒时间和死亡率的影响,并检测治疗性给药对小鼠血清乙醇浓度和肝、胃组织乙醇脱氢酶(ADH)活性的影响。结果:与模型组比较,荆防败毒散加减组预防性给药可降低小鼠醉酒率(P<0.05),明显延长醉酒潜伏期(P<0.01);与模型组比较,荆防败毒散加减治疗性给药可降低小鼠死亡率(P<0.05或P<0.01),缩短醉酒时间(P<0.05或P<0.01)。与正常组比较,模型组小鼠血清乙醇浓度升高(P<0.05),肝和胃组织ADH活性降低(P<0.05或P<0.01)。与模型组比较,荆防败毒散加减组均可降低小鼠血清乙醇浓度(P<0.05),升高肝和胃组织ADH活性(P<0.05或P<0.01)。结论:荆防败毒散加减通过升高急性酒精中毒小鼠肝组织和胃组织ADH活性,促进酒精代谢,降低血乙醇浓度,具有显著解酒促醒作用。

Alleviating Action of Jingfang Baidu San on Acute Alcoholism in Mise

Objective: To observe the alleviating effects of Jingfang Baidu San on acute alcoholism in mouse. Method: The mice were randomly divided into five groups, including normal,group, model group, Jingfang Baidu San high,moderate,low-dose group( the dose was calculated by the amount of crude drug 32.4,16.2,8.1 g·kg^-1,and ig given before or after 30 minutes of drinking alcohol).Except normal group, the model of acute alcoholism was established by drinking 14 mL·kg^-1 56° Hongxing Erguotou alcohol once. The rate and time of alcohol drink were observed after preventing treatment, and the death rate and sober-up time were observed after treatmen. Alcohol density in serum and the activity of 14 mL·kg^-1(ADH) in liver and stomach tissue were observed. Result: Compared with model group,the rate of alcohol drink in Jingfang Baidu San group was decreased (P<0.05),and the time of alcohol drink was longer(P<0.01). The death rate in Jingfang Baidu San group was decreased(P<0.05 orP<0.01), and sober-up time was shorter (P<0.05 orP<0.01) after treatment.Compared with normal group.The alcohol density in serum was increased and the activity of ADH in liver and stomach tissuedecreased in model group (P<0.01). Compared with model group,the alcohol density in serum of each group were decreased(P<0.01),the activity of ADH in liver and stomach tissue in Jingfang Baidu San group were increased(P<0.05 orP<0.01).Conclusion Jingfang Baidu San can improve the activity of ADH in liver and stomach tissue in acute mouse alcoholism,and promoting alcohol metabolism to reduce alcohol density, so Jingfang Baidu San can alleviate acute alcoholism in mise effectively.