天麻饮片的HPLC指纹图谱鉴别

目的:研究建立天麻饮片的HPLC指纹图谱鉴别方法。方法:选择HPLC-UV指纹图谱法,Kromasil C18柱;流动相乙腈-0.5%冰醋酸溶液线形梯度洗脱,检测波长270 nm,流速1.0 mL.min-1。以指纹图谱软件计算相似度和图谱比较,以聚类分析比较不同来源饮片的差异。结果:建立了天麻饮片的标准指纹图谱,6个产地共10批次天麻饮片的指纹图谱基本一致,共能检出15个共有峰,10个样品的平均相似度>98%,但相对峰面积有较大差异;聚类分析结果与饮片产地基本一致。结论:该方法重复性好,结合聚类分析可用于天麻饮片的定性鉴别,为天麻饮片的质量控制提供了科学依据。

HPLC Fingerprint Chromatograms for Processed Products of Gastrodia elata

Objective: To establish the HPLC fingerprint chromatogram identification of the processed products from Gastrodia elata . Method: HPLC procedure was performed on a chromatographic column of Kromasil C₁₈. Acetonitrile-glacial acetic acid water solution(0.5%)was used as mobile phase by gradient elution, and the detection wavelength was at 270 nm. The mobile phase flow rate was at 1.0 mL·min^-1. The similarity and the clustering analysis were carried out by fingerprint chromatogram software. Result: The standard HPLC Fingerprint chromatogram was established through the determination of 10 batches processed products from 6 different producing areas. 15 peaks can be detected and the average similarity was over 98% in 10 samples. The processed products were almost the same on the whole chromatograms but the relative peak areas had the differences. The cluster analysis was consistent with the processed origins. Conclusion: HPLC method can be used in quality control of G. elata .