| 中药多糖与过继免疫联合治疗卵巢癌 |
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| 引用本文： | 许云霞,刘晓健,屈飞.中药多糖与过继免疫联合治疗卵巢癌[J].中国实验方剂学杂志,2011,17(21):231-234. |
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| 作者姓名： | 许云霞 刘晓健 屈飞 |
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| 作者单位： | 1. 江苏大学附属人民医院妇产科,江苏镇江,212002
2. 江西中医学院药学院药理学教研室,南昌,330004 |
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| 摘 要： | 目的:观察比较中药多糖干预后的多种细胞因子诱导的杀伤细胞(CIK)细胞在不同条件下特异性杀伤卵巢癌SKOV3细胞的效果,探讨中药与过继免疫联合治疗卵巢癌的可行性。方法:实验分4组,CIK组、PBOC-CIK组、SKOV3Ag-PBDC-CIK组及SKOV3Ag-ADC-CIK组。联合猪苓多糖(100 mg.L-1)、茯苓多糖(100 mg.L-1)、黄芪多糖(100 mg.L-1)及多种细胞因子对卵巢癌患者外周血CIK细胞进行体外诱导和扩增,以卵巢癌细胞株SKOV3冻融抗原致敏外周血与腹水来源的DC细胞,比较单纯CIK、未经抗原负载的外周血树突状细胞(DC)活化的CIK、经抗原负载的外周血DC活化的CIK、经抗原负载的腹水DC活化的CIK对SKOV3细胞的杀伤作用,并动态观察CIK细胞的增殖情况。结果:①CIK与DC共培养可显著增加CIK增殖倍数(P<0.01);②中药多糖干预后CIK对SKOV3卵巢癌细胞株有明显杀伤作用,且杀伤率随效靶比增加而提高,细胞杀伤率分别为(9.12±0.21)%,(10.15±0.27)%,(11.20±0.34)%和(12.73±0.43)%(P<0.05);③SKVO3冻融抗原负载的外周血DC可显著增加CIK特异性杀伤卵巢癌SKOV3细胞的能力,杀伤率显著高于未负载抗原外周血DC活化的CIK细胞和单纯CIK细胞(P<0.05或P<0.01);④腹水来源DC负载抗原后所活化的CIK细胞对SKOV3细胞杀伤率,与负载抗原的外周血来源DC活化的CIK无明显差异。结论:经SKOV3肿瘤细胞冻融抗原冲击致敏的DC,可促进中药多糖干预后CIK细胞扩增,增强CIK细胞对SKOV3卵巢癌细胞的杀伤作用,且卵巢癌腹水可作为过继免疫治疗中效应细胞的一个重要来源。
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| 关 键 词： | 卵巢肿瘤 CIK细胞 树突细胞 中药多糖 细胞毒性 |
| 收稿时间： | 2011/6/21 0:00:00 |
Effects of Chinese Medicine Polysaccharides Combined with Adoptive Immunotherapy on Ovarian Cancer |
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| XU Yun-xi,LIU Xiao-jian and QU Fei.Effects of Chinese Medicine Polysaccharides Combined with Adoptive Immunotherapy on Ovarian Cancer[J].China Journal of Experimental Traditional Medical Formulae,2011,17(21):231-234. |
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| Authors: | XU Yun-xi LIU Xiao-jian and QU Fei |
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| Institution: | Department of Gynecology and Obstetrics, People Hospital Affiliated to Jiangsu University, Zhenjiang 212002, China;Department of Gynecology and Obstetrics, People Hospital Affiliated to Jiangsu University, Zhenjiang 212002, China;Department of Pharmacology, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China |
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| Abstract: | Objective:To study the killer activity of cytokine-induced killer(CIK) cells treated with Chinese medicine polysaccharides against the ovarian cancer cell line SKOV3 under different conditions,and explore the possibility of adoptive immunotherapy combined with Chinese medicine in the treatment of ovarian cancer.Method: The experiment were divided into four groups,such as CIK group,PBDC-CIK group,SKOV3Ag-PBDC-CIKgroup and SKOV3Ag-ADC-CIKgroup.CIK cells derived from the peripheral blood(PB) were induced by cy... |
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| Keywords: | ovarian neoplasms CIK cell dendritic cells Chinese medicine polysaccharides cytotoxicity |
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