大孔吸附树脂纯化愈肾颗粒中总黄酮工艺

目的:研究大孔吸附树脂法纯化愈肾颗粒总黄酮最佳工艺。方法:以总黄酮的比吸附量、解析率、洗脱率等为指标,选用7种大孔吸附树脂对愈肾颗粒总黄酮进行纯化。结果:HPD100型大孔吸附树脂对愈肾颗粒的总黄酮具有较好的分离纯化能力。最佳工艺条件为2 BV的愈肾颗粒供试品溶液为上样量(含生药量为1 g.mL-1),吸附速率为1 BV.h-1,分别用水2BV、30%乙醇3 BV、50%乙醇4 BV洗脱,洗脱流速4 BV.h-1,合并30%乙醇和50%乙醇洗脱液,浓缩,即为纯化的总黄酮。结论:纯化后总黄酮含量提高到76%以上,HPD100型大孔吸附树脂可较好的纯化愈肾颗粒总黄酮。

Purification Technology of Total Flavonoids from Yushen Granules by Macroporous Resin

Objective: To study on optimum purification technology of total flavonoids from Yushen granules by macroporous resin. Method: With adsorption capacity, desorption rate and elution rate as indexes, purified total flavonoids of Yushen granules by seven macroporous resin. Result: HPD100 macroporous resin showed good separation and purification capabilities of total flavonoids from Yushen granules.The optimum technology conditions were as follows:2 BV sample(dose of raw drug was 1 g·mL^-1) of Yushen granules formula was passed through the column with flow rate of 1 BV·h^-1, eluted by 2 BV water, 3 BV 30% ethanol, 4 BV 50% ethanol respectively, elution flow rate was 2 BV·h^-1.Combined 30% and 50% ethanol elutes were concentrated to yield the purification of total flavonoids. Conclusion: The purity of total flavonoids was up to 76%. HPD100 could be well used in purifying total flavonoids from Yushen granule.