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| HPLC同时测定灯盏细辛注射液中6种主要成分的含量 | |
| 引用本文： | 周玲,谢丽艳,徐洁,居文政,张军,谈恒山.HPLC同时测定灯盏细辛注射液中6种主要成分的含量[J].中国实验方剂学杂志,2011,17(21):78-81. |
| 作者姓名： | 周玲 谢丽艳 徐洁 居文政 张军 谈恒山 |
| 作者单位： | 1. 南京中医药大学药学院,南京,210009 2. 南京中医药大学附属医院临床药理科,南京,210029 3. 南京军区总医院,南京,210002 |
| 基金项目： | 国家重大新药创制项目(2008ZX09312-004);江苏省中医药领军人才项目(LJ200906) |
| 摘 要： | 目的:建立HPLC同时测定灯盏细辛注射液中绿原酸、咖啡酸、1,3-O-二咖啡酰奎宁酸、灯盏花乙素、3,5-二-O-咖啡酰奎宁酸、4,5-二-O-咖啡酰奎宁酸含量的方法。方法:Agilent Zorbax SB-C18(4.6 mm×150 mm,5μm)色谱柱,流动相乙腈-0.1%甲酸水溶液,梯度洗脱,柱温30℃,检测波长327 nm,流速1.0 mL.min-1,进样量20μL。结果:绿原酸、咖啡酸、1,3-O-二咖啡酰奎宁酸、灯盏花乙素、3,5-二-O-咖啡酰奎宁酸、4,5-二-O-咖啡酰奎宁酸的线性范围分别为0.079～5.065(r=0.999 9),0.080～5.120(r=0.999 9),0.074～4.750(r=0.999 9),0.391～25.050(r=0.999 9),0.038～2.423(r=0.999 9),0.487～7.792 mg.L-1(r=0.999 8),加样回收率分别为96.72%(RSD 1.76%),96.64%(RSD 1.69%),101.81%(RSD 1.81%),98.16%(RSD 2.22%),99.02%(RSD 2.57%),97.02%(RSD 1.52%)。结论:方法简便、快捷,准确、重复性好,可为灯盏细辛注射液提供质量控制依据。 |
| 关 键 词： | 灯盏细辛注射液 绿原酸 咖啡酸 1 3-O-二咖啡酰奎宁酸 灯盏花乙素 3 5-二-O-咖啡酰奎宁酸 4 5-二-O-咖啡酰奎宁酸 |
| 收稿时间： | 5/3/2011 12:00:00 AM |
Simultaneous Determination of Six Major Compounds in Dengzhanxixin Injection by HPLC |
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| ZHOU Ling,XIE Li-yan,XU Jie,JU Wen-zheng,ZHANG Jun and TAN Heng-shan.Simultaneous Determination of Six Major Compounds in Dengzhanxixin Injection by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2011,17(21):78-81. | |
| Authors: | ZHOU Ling XIE Li-yan XU Jie JU Wen-zheng ZHANG Jun and TAN Heng-shan |
| Institution: | Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China;Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China;Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China;Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China;Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China;General Hospital of Armed Forces of Nanjing, Nanjing 210002, China |
| Abstract: | |
| Keywords: | Dengzhanxixin injection chlorogenic acid caffeic acid 1 3-O-dicaffeoylquinic acid scutellarin 3 5-dicaffeoylquinic acid 4 5-dicaffeoylquinic acid |
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