养心调肝益肾煎剂对去势雄性大鼠的壮阳作用

目的:研究养心调肝益肾煎剂壮阳作用及其可能机制。方法:手术去势造模,造模后第8天对不同实验组分别ig生理盐水(NS)、养心调肝益肾煎剂高、低剂量(50,25 g.kg-1)、男宝混悬液(0.56 g.kg-1),连续用药21 d后测量去势雄鼠附性器官包皮腺、精液囊-前列腺、提肛肌的质量,计算其脏器系数,测量血清睾酮(T)、皮质醇(F)及阴茎海绵体内皮细胞一氧化氮(NO)的含量。结果:养心调肝益肾煎剂组与模型组比较,有明显增加去势雄鼠包皮腺、精液囊-前列腺、提肛肌脏器系数及血清T、F及阴茎NO含量的作用(P<0.05),尤以养心调肝益肾煎剂高剂量组更为明显(P<0.05)。结论:养心调肝益肾煎剂可调节下丘脑-垂体-性腺轴及肾上腺皮质系统功能,具有壮阳作用。

Strengthening Yang Action of Yangxin Tiaogan Yishen Decoction in Catrated Male Rats

Objective: To investigate strengthening yang action of Yangxin Tiaogan Yishen decoction and its possible mechanism. Method: On the 8^th days after making castrated model rats by operation,we respectively fed NS,Yangxin Tiaogan Yishen decoction(50,25 g ·kg^-1),Nanbao mixed liquid(0.56 g ·kg^-1) into the stomaches of the different groups of male rats for 21 days. Afterwards,the weight of prepputial gland,sperm bladder-prostate,anus levator muscle,content of testosterone(T) and cortisol(F) in blood serum and nitric oxide(NO) content of endothelium cell of penial corpus cavernosum were observed. Result: The indexes of prepputial gland,sperm bladder-prostate,anus levator muscle,and content of T,F and NO were increased,these items of Yangxin Tiaogan Yishen decoction group were higher than these of the model group (P<0.05). The deta in Yangxin Tiaogan Yishen decoction with high dose group were especially remarkable(P<0.05). Conclusion: Yangxin Tiaogan Yishen decoction can adjust lower thalamus-hypophysis-sexual gland axis and adrenal function,and it can strengthen yang action.