Doehlert设计法优化紫珠中苯乙醇苷的提取工艺

目的:研究乙醇回流法提取枇杷叶紫珠中苯乙醇苷的优化工艺。方法:以料液比、提取时间、乙醇体积分数为3个考察因素,苯乙醇苷含量为指标,运用响应面分析法中的Doehlert设计法进行试验设计,并采用统计学和数学软件对试验数据进行分析,拟合出苯乙醇苷类的最优提取工艺条件。结果:优选的提取条件为以12倍量90%乙醇回流提取2次,每次2 h。结论:应用Doehlert设计的响应面优化法具有使用方便、预测性好的特点,值得推广应用。

Optimization of Extraction Technology of Phenylethanoid Glycoside from Callicarpa konchiana by Doehlert Design

Objective: To optimize the process of phenylethanoid glycoside from Callicarpa konchiana by reflux ethanol extraction. Method: Three factors were investigated by Doehlert design with the content of phenylethanoid glycoside as criterias, including the ratio of material to liquid,extracting time and the alcoholic concentration. And statistics and mathematics software were used to analyze the test data,then prepared the optimum extraction process. Result: The optimum extraction conditions were as follows: reflux extraction with 12-fold 90% alcohol for 2 times, 2 hours each time. Conclusion: The doehlert design is easy to use and has good predictability, it can be widely applied in process optimization.