| 高效毛细管电泳法测定三棵针中3种生物碱的含量 |
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| 引用本文： | 胡成刚,高言明,王柳卜,孙济平,贾宪生. 高效毛细管电泳法测定三棵针中3种生物碱的含量[J]. 中国实验方剂学杂志, 2011, 17(18): 71-74 |
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| 作者姓名： | 胡成刚 高言明 王柳卜 孙济平 贾宪生 |
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| 作者单位： | 贵阳中医学院,贵阳,550002 |
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| 基金项目： | 贵州省中药现代化专项基金(黔科合社字[2009]5016号) |
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| 摘 要： | 目的:建立毛细管区带电泳同时测定不同产地三棵针中盐酸小檗碱、盐酸药根碱、盐酸巴马汀含量的方法。方法:空心石英毛细管(75 cm×50μm)柱,以50 mmol.L-1硼砂-甲醇(2∶1)的溶液为电泳介质,运行电压25 kV,柱温15℃,检测波长230 nm。结果:盐酸小檗碱、盐酸药根碱及盐酸巴马汀分别在0.816 8～32.672 0 mg.L-1(r2=0.999 4),0.852 0～8.520 0 mg.L-1(r2=0.999 5),0.815 2～8.152 0 mg.L-1(r2=0.999 6)线性关系良好;其平均加样回收率分别为103.8%(RSD 1.14%),100.7%(RSD 3.46%),100.2%(RSD 2.87%)。结论:实验方法快速简便,准确可靠,适用于三棵针等药材中盐酸小檗碱、盐酸巴马汀及盐酸药根碱的含量测定。
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| 关 键 词： | 毛细管电泳法 三棵针 盐酸小檗碱 盐酸药根碱 盐酸巴马汀 |
| 收稿时间： | 2011-04-15 |
Simultaneous Determination of Three Alkaloids in Sankezhen by High Performance Capillary E1ectrophoresis |
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| HU Cheng-gang,GAO Yan-ming,WANG Liu-bu,SUN Ji-ping and JIA Xian-sheng. Simultaneous Determination of Three Alkaloids in Sankezhen by High Performance Capillary E1ectrophoresis[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(18): 71-74 |
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| Authors: | HU Cheng-gang GAO Yan-ming WANG Liu-bu SUN Ji-ping JIA Xian-sheng |
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| Affiliation: | Department of Pharmacy Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;Department of Pharmacy Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;Department of Pharmacy Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;Department of Pharmacy Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;Department of Pharmacy Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China |
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| Abstract: | |
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| Keywords: | High Performance Capillary E1ectrophoresis Sankezhen berberine hydrochloride jatrorrhizine hydrochloride palmatine hydrochloride |
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