HPLC法同时测定复方樟脑搽剂中苯酚和樟脑含量

目的建立同时测定复方樟脑搽剂中苯酚和樟脑含量的HPLC法。方法色谱柱为Kromasil-C18（150mm×4．6mm，5μ m）；流动相为甲醇-水（55：45）；检测波长287nm；流速0．8ml／min。结果苯酚和樟脑的线性范围分别在103．4～827．2μg／ml（r=0．9991，n=5）、205．1-1640．8μg／ml（r=0．9995，n=5），平均回收率分别为99．61％（RSD=1．20％，n=9）、99．83％（RSD=1．18％，n：9）。结论该方法简便、准确，重复性好，适用于复方樟脑搽剂的质量控制。

Simultaneous determination of phenol and camphor in compound camphor liniment by HPLC

Objective To establish a method for simultaneous determination of phenol and camphor in compound camphor liniment by HPLC. Methods The separation was performed on a Kromasil C18 analytical column（ 150 mm × 4.6 mm, 5 μm）with the mobile phase consisting of methanol-water（55 ： 45）. The detection wavelength was 287 nm. The flow rate was 0.8 ml/min. Results The standard curves were linear in the range of 103.4 - 827.2μg/ml for phenol （ r = 0. 999 1, n = 5 ） and 205.1 - 1 640.8μg/ml（ r = 0. 999 5, n = 5 ）for camphor. The average recoveries of phenol and camphor were 99.61% （RSD = 1. 19% , n = 9 ） and 99.83 % （RSD = 1. 18% ,n = 9）. Conclusion The method was simple and accurate with a good reproducibility, and could be used as a quality control method for compound camphor liniment.