参龙健脑胶囊对脑缺血损伤大鼠海马区胆碱 乙酰基转移酶表达的影响

目的:探讨参龙健脑胶囊对脑缺血损伤大鼠脑保护作用及对海马区胆碱乙酰基转移酶(ChAT)表达的影响。方法:采用结扎双侧颈总动脉制备脑缺血模型,参龙健脑胶囊低、高剂量组和吡拉西坦组剂量分别为0.4,1.2,0.45 g.kg-1,ig 1次/d。ig 28 d后观察神经功能并评分、脑病理组织切片并应用免疫组织化学染色法观察ChAT在缺血脑损伤大鼠海马区的表达。结果:模型组大鼠神经功能缺损明显(P<0.05),海马区ChAT表达减少(P<0.01);与模型组相比,参龙健脑胶囊治疗组大鼠的神经功能缺损改善(P<0.05),大鼠海马区ChAT表达显著增加(P<0.01,P<0.05)。结论:参龙健脑胶囊对脑组织损伤的保护作用与其促进脑组织ChAT表达密切相关。

Effect of Shenlong Jiannao Capsule on Expression of Choline Acetyltransferase in Hippocampus of Cerebral Ischemia Rats

Objective: To explore the effects of Shenlong Jiannao capsule on the expression of choline acetyl transferase(ChAT) in hippocampus of cerebral ischemia rats. Method :Two-vessel occlusion were adopted to reproduce the cerebral ischaemia animal model.Rats were randomly divided into 5 groups as following: sham operation group, model control group,positive control group with pisacetam,Shenlong Jiannao capsule groups(low and high dosage). Shenlong Jiannao capsule groups low and high dosage treated by gavage with Shenlong Jiannao capsule at the dosage of 0.4,1.2 g·kg^-1·d^-1. After 28 days,changes of neurological scores and pathological tissue sections of brain in rats were observed,immunohistochemistry were adopt used to assay the expression of ChAT in hippocampus of cerebral ischaemia rats. Result: Shenlong Jiannao capsule could improve the neurological scores, ameliorate the morphology and increase the number of ChAT immunoreactive positive neurons.Comparing to model control group, the neurological scores were improved(P<0.05),the expression of ChAT was increased in Shenlong Jiannao capsule group(P<0.01, P<0.05). Conclusion: Shenlong Jiannao capsule can ameliorate the cerebral injury involved in enhancing the ChAT protein expression.