| 大鼠腹腔肥大细胞与RBL-2H3细胞脱颗粒方法的 建立及其在中药注射剂安全性评价上的应用 |
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| 引用本文： | 李咏梅,刘炯,赵源,汤家铭.大鼠腹腔肥大细胞与RBL-2H3细胞脱颗粒方法的 建立及其在中药注射剂安全性评价上的应用[J].中国实验方剂学杂志,2011,17(22):152-157. |
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| 作者姓名： | 李咏梅 刘炯 赵源 汤家铭 |
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| 作者单位： | 上海中医药大学实验动物中心,上海,201203 |
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| 基金项目： | 科技部重大新药创制基金项目(2009ZX09502-002) |
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| 摘 要： | 目的:建立大鼠体外肥大细胞以及RBL-2H3细胞脱颗粒试验的方法,评估2种细胞脱颗粒方法对中药注射剂(traditional Chinese medicine injections,TCMI)引起脱颗粒的可行性。方法:提取大鼠腹腔肥大细胞(rat peritoneal mast cell,RPMC),优化实验条件,用阳性药物C48/80和各种TCMI等与其共培养,通过甲苯胺蓝染色法计数腹腔MC脱颗粒率;通过RBL-2H3细胞与TCMI共同培养,底物显色法检测β-氨基己糖苷酶释放率。结果:阳性对照品C48/80的浓度与RPMC脱颗粒有一定的量效关系;参麦、冠心宁、痰热清、清开灵和生脉5种TCMI能显著引起细胞脱颗粒,并与药物浓度有一定的正相关性;RBL-2H3细胞脱颗粒方法中参麦、黄芪、银杏、痰热清、丹香冠心、清开灵和生脉7种TCMI能是β-氨基己糖苷酶释放率升高。结论:一些TCMI能引起体外RPMC和RBL-2H3细胞脱颗粒,2种细胞脱颗粒方法在评价一些TCMI可能引起类过敏反应方面具有一定的互补性与可行性。
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| 关 键 词： | 类过敏反应 中药注射剂 肥大细胞脱颗粒 RBL-2H3细胞 β-氨基己糖苷酶释放率 |
| 收稿时间： | 2011/4/27 0:00:00 |
Studies on Degranuation of Rat Peritoneal Mast Cells and RBL-2H3 Cells Caused by Traditional Chinese Medicine Injections |
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| LI Yong-mei,LIU Jiong,ZHAO Yuan and TANG Jia-ming.Studies on Degranuation of Rat Peritoneal Mast Cells and RBL-2H3 Cells Caused by Traditional Chinese Medicine Injections[J].China Journal of Experimental Traditional Medical Formulae,2011,17(22):152-157. |
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| Authors: | LI Yong-mei LIU Jiong ZHAO Yuan and TANG Jia-ming |
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| Institution: | Laboratory Animal Center, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Laboratory Animal Center, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Laboratory Animal Center, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Laboratory Animal Center, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| Abstract: | Objective: Evaluating the feasibility of the degranulation methods in rats mast cells in vitro and in RBL-2H3 cells in vitro caused by traditional Chinese medicine injections(TCMI).Method: Mast cells were gathered from rats peritoneal,the experimental conditions were optimized,the positive drug C48/80 and various TCMI were incubated to observe rat peritoneal mast cells degranulation caused by TCMI,through counting the degranulation rate of rat peritoneal mast cells whitch staining by toluidine blue.The posi... |
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