加味四逆散对异相睡眠剥夺大鼠前额叶 皮质突触可塑性改变的研究

目的:探讨加味四逆散对异相睡眠剥夺168 h大鼠的学习记忆能力及前额叶皮质突触结构可塑性的作用。方法:以多平台法复制睡眠剥夺模型,药物干预组以加味四逆散灌胃,分别在睡眠剥夺6,30,54,78,102,126,150 h时间点给药,每次用药量为16.25 g.kg-1。应用Y迷宫评估动物学习与记忆能力的变化,并使用透射电镜观察动物前额叶皮质(prefrontalcortex,PFC)突触结构可塑性的改变。结果:加味四逆散组大鼠Y迷宫成绩明显高于模型组大鼠(P<0.05)。与模型组比较,加味四逆散组大鼠PFC神经元突触间隙明显变窄(P<0.05),突触后膜致密物(post synaptic density,PSD)厚度明显变厚(P<0.01)。结论:加味四逆散能改善异相睡眠剥夺模型大鼠学习与记忆能力,与其影响前额叶皮质神经元突触结构可塑性,从而增强其突触传递效能有关。

Effect of Jiawei Sini Powder on Learning and Memory in Sleep Deprived Rats

Objective: To study the effects of Jiawei Sini powder on learning and memory ability and synaptic plasticity in prefrontal cortex of sleep deprived rats. Method: Experimental model of sleep deprivation was established by use of multi-platform method. The intervention group were fed with Jiawei Sini powder, and the dosage is 16.25 g·kg^-1. The variation of learning and memory was detected by Y maze method at pre and post sleep deprivation.The changes of the ultrastructure of rat prefrontal cortex were observed by using transmission electron microscopy. Result: The percentage of correct responses of intervention group was significantly higher than those in model group (P<0.05) .Compared with the model group, the post synaptic density (PSD) in prefrontal cortex areas in the intervention group was significantly thickened (P<0.01) . Conclusion: Jiawei Sini powder could improve the ability of learning and memory in sleep deprived rats via increasing synaptic plasticity in prefrontal cortex.