外源生长素对黄芩悬浮细胞有效成分和内源激素含量的影响

目的:探讨外源生长素对黄芩悬浮细胞有效成分和内源激素含量的影响。方法:将稳定继代的黄芩愈伤组织在含有不同浓度NAA或KT的MS液体培养基悬浮震荡培养,光照16 h黑暗8 h进行培养18 d后,采用HPLC的方法,测定黄芩苷的含量,采用酶联免疫吸附法测定内源激素的含量,以黑暗条件为对照。结果:①与对照组相比,光照和黑暗条件下分别加入0.2 mg.L-1NAA后,黄芩苷含量显著降低,分别为未加入NAA时的2.56%,3.36%。②黑暗条件下,加入外源NAA后,ZR含量显著降低,但IAA,ABA和GAs含量变化不明显。③0.2 mg.L-1 NAA和不同浓度KT的协同作用对黄芩苷的含量呈现先增长后降低的趋势。其中1.0 mg.L-1的KT作用下黄芩苷含量最高,且黑暗条件下显著高于光照条件,达到2.13μg.L-1。结论:外源生长素不利于黄芩有效成分黄芩苷的积累,0.2 mg.L-1 NAA+1.0 mg.L-1 KT的组合对黄芩苷的积累量具有显著的促进作用。

Effects of External Auxin on Effective Components and Endogenous Hormones Contents in Scutellaria.baicalensis Suspension Cell

Objective: To study the effects of auxin on the active compounds and endogenous hormones in Scutellaria baicalensis suspension cell cultures. Method: The subculture scutellariae callus were cultivated in MS liquid medium containing various concentration of NAA or KT on a gyratory shaker with a 16-h photoperiod. After 18 d, the contents of baicalin and endogenous hormones were determined with HPLC and enzyme-linked immunosorbent assay, respectively. Darkness condition was as control. Result: (1) After 0.2 mg ·L^-1 NAA treatment, the content of baicalin was 2.56% and 3.36% lower than of untreatment, respectively. (2) After NAA treatment on the dark conditions, the content of ZR was significantly reduced. (3) The synergistic effect of 0.2 mg ·L^-1 NAA with different concentration of KT on the content of baicalin presented the trend of rising then declined. And after 1.0 mg ·L^-1 KT treatment, the content of baicalin was the highest levels on the dark conditions and significantly higher than light condition. Conclusion: Auxin is not suitable for the active compound production of S. baicalensis. The combination of 0.2 mg ·L^-1 NAA+1.0 mg ·L^-1 KT promotes the accumulation of baicalin.