槐定碱对内毒素致急性肺损伤小鼠免疫调节及抗氧化的影响

目的:探讨槐定碱对内毒素致急性感染小鼠的免疫调节及氧化损伤保护作用。方法:采用随机数字法,将昆明小鼠60只分为6组:正常组、模型组、预防给药组、槐定碱低、中、高剂量组,每组10只。预防给药组连续6 d ip槐定碱5 mg.kg-1,其余各组ip 0.5 mL/只生理盐水,均末次给药后1 h除A组外其他各组一次性ip内毒素9 mg.kg-1建立小鼠急性感染模型。造模后2 h,槐定碱低、中、高剂量组分别ip槐定碱2.5,5,9 mg.kg-1,观察小鼠行为学变化;进行肺含水量的测定;取血检测白介素6(IL-6)水平和超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)的变化;HE染色观察肺组织细胞的形态改变。结果:给槐定碱各组小鼠的急性感染程度较模型组有明显改善,血清IL-6,MDA,NO含量较模型组降低(P<0.05);SOD含量较模型组升高(P<0.05);中剂量组效果优于其他各组(P<0.05)。HE染色提示,各给药组炎症程度均较模型组有不同程度的改善。结论:槐定碱可不同程度的减轻内毒素肺损伤小鼠的病理损害,能有效地增强模型鼠免疫调节能力及抗氧化损伤能力。

Immune Adjustment and Antioxidant Effect of Sophoridine on Acute Lung Injury Induced by Lipopolysaccharide in Mice

Objective: To investigate effects of sophoridine on immune and oxidative injury of acute infection induced by endotoxinin mice. Method: Sixty Kunming mice were randomly divided into 6 groups: normal group, model group, preventive treatment group, low dose, midium dose, and high dose of sophoridine group (n=10, each). Mice in preventive treatment group were injected with 5 mg ·kg^-1 sophoridine ip continuously for 6 days, the mice in the rest groups were ip injected 5 mg ·kg^-1 saline. One hour after the last administration, mice in all groups except those in group A were ip given endotoxin (9 mg ·kg^-1 )to induce acute infection. After 2 h of modeling, sophoridine groups were given ipsophoridine 2.5, 5, 9 mg ·kg^-1 accordingly, the changes in mice were observed; including lung water content; serum interleukin-6 (IL-6), superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO). Cellular changes in lung tissue was observed by HE staining. Result: Mice treated by sophoridine in each group showed significant improvement in the degree of acute infection, and serum IL-6, MDA, NO levels were lower than those in model group (P<0.05); SOD level was higher than that in model group (P<0.05). Nedium-dosage group was better than the other groups (P<0.05). HE staining showed that the degree of inflammation of the treatments resulted in different degrees of improvement compared with the model group. Conclusion: Sophoridine can reduce the pathological damage in model mice with endotoxin injury to certain degree,and effectively enhance the immune function and the anti-oxidative ability.