复方党参提取物对环磷酰胺处理小鼠免疫功能的调节作用

目的:观察复方党参提取物对环磷酰胺所致免疫低下小鼠免疫功能的调节作用。方法:小鼠随机分为对照组、模型组和复方党参提取物低、中、高剂量组(0.25,0.5,1.0 g.kg-1),各剂量组、对照组和模型组均ig给药,持续10 d,并在第7天开始同时ip环磷酰胺制作免疫抑制小鼠模型。MTT法检测免疫细胞的活力,鸡红细胞吞噬检测巨噬细胞的吞噬功能。绵羊红细胞免疫法检测小鼠体液免疫功能。结果:复方党参提取物各组脾淋巴细胞转化率升高、足跖厚度增加;脾细胞溶血空斑数增加、血清溶血素水平及腹腔巨噬细胞吞噬率增加;复方党参提取物中、高剂量组与模型组比较有非常显著性差异(P<0.01)。结论:复方党参提取物对环磷酰胺处理的免疫低下小鼠有免疫保护作用。

Immunomodulative Effect of Compound Codonopsis Radix Extract on Immunosuppressive Mice Induced by Cyclophosphamide

Objective: To observe the effect of compound Codonopsis Radix extract on immunological function in immunosuppressive model mice induced by cyclophosphamide. Method: The immunosuppressive model in mice was induced by cyclophosophamide. Mice were administered with (low, medium and high dose, 0.25,0.5 and 1.0 g ·kg^-1 accordingly) compound Codonopsis Radix and control groups were administered with distilled water for 10 days. MTT assay was employed to evaluate the metabolic activity of splenocytes.The changes in the phagocytotic activity of macrophage in response to compound Codonopsis Radix extract treatment were tested by phagocytosis percentage of chicken red blood cells (CRBCs).The effect of compound Codonopsis Radix extract on humoral immunity was assessed in mice immunized with sheep red blood cells (SRBCs) by measuring the serum levels of specific antibody against SRBCs. Result: Compound Codonopsis Radix extract significantly increased the paw swelling in mice, enhanced the metabolic activity of mouse splenocytes, and also significantly increased serum hemolysin level immunized with SRBCs and capacity of mouse peritoneal macrophages for CRBC phagocytosis in mice induced by cyclophosophamide. Conclusion: Compound Codonopsis Radix extract can stimulate the immunologic function in immunosuppressive mice.