厚朴枳实配伍对大鼠胃动力的影响

目的:考察厚朴枳实配伍及不同煎煮时间对大鼠胃动力的影响。方法:SD大鼠ip左旋精氨酸(L-Arg)6 d建立大鼠胃动力异常模型。胃动力异常大鼠连续5 d分别ig给予厚朴提取物(10 g.kg-1)、厚朴枳实配伍提取物(20 g.kg-1),ip西沙必利(0.004 2 g.kg-1)、观察其对L-Arg所致胃动力异常大鼠胃动力的改善作用。结果:大鼠ip L-Arg造成大鼠胃动力减缓、血浆胃动素减少。厚朴、厚朴枳实配伍提取液能有效抑制L-Arg引起的大鼠胃动力下降(P<0.05)和胃动素的下降(P<0.05),厚朴与阳性药物西沙必利之间无显著性差异,厚朴枳实配伍与厚朴、西沙必利之间有显著性差异(P<0.05)。厚朴、厚朴枳实配伍、西沙必利组的胃残留率分别为(69.3±13.7)%,(60.7±12.9)%,(70.1±12.9)%,;胃动素分别为(85.4±11.2),(93.1±10.4),(86.3±12.4)nmol.L-1。厚朴枳实煎煮90 min较煎煮60,30 min大鼠胃残留率低(P<0.05),胃动素含量高(P<0.05)。煎煮90,60,30 min胃残留率分别为(47.7±12.8)%,(59.8±12.1)%,(62.7±11.8)%;胃动素分别为(102.3±10.6),(92.9±10.1),(89.6±11.2)nmol.L-1。结论:厚朴、厚朴枳实配伍提取液能显著改善L-Arg所致的大鼠胃动力障碍,厚朴枳实配伍后作用要优于单一厚朴。两者配伍以煎煮90 min为宜。

Influence of Magnoliae Officinalis Cortex Compatibility of with Aurantii Frutus Immaturus on Gastric Motility in Rats

Objective: To investigate the influences of compatibility of Magnoliae Officinalis Cortex with Aurantii Frutus Immaturus on gastric motility in rat. Method: The gastric motility disorder in SD rats was established by injecting L-Arg intraperitoneally for six days, then the rats were given the extract of Magnolia (Magnoliae Officinalis Cortex 10 g ·kg^-1) by intragastric administration, the compatibility of extract of Magnoliae Officinalis Cortex with Frutus auranti (Magnoliae Officinalis Cortex and Aurantii Frutus Immaturus 20 g ·kg^-1) by intragastric administration, cisapride (0.004 2 g ·kg^-1) by intraperitoneal injection, respectively. The improvement in gastric function was observed. Result: Gastrointestinal motility in rats injected with L-Arg intraperitoneally was slowed down, plasma motilin was reduced. The extract of Magnoliae Officinalis Cortex, Magnoliae Officinalis Cortex compatibility with Aurantii Frutus Immaturus could effectively inhibit the decrease in gastrointestinal motility (P<0.05) and plasma motilin (P<0.05). and there was significant difference between the group Magnoliae Officinalis Cortex and the group Magnoliae Officinalis Cortex compatibility with Aurantii Frutus Immaturus (P<0.05) and there was no significant difference between Magnoliae Officinalis Cortex and the positive drug cisapride. There were significant differences between the group Magnoliae Officinalis Cortex compatibility with Aurantii Frutus Immaturus compared with the group Magnoliae Officinalis Cortex, and the group of compatibility compared with cisapride (P<0.05). Gastric residual rate in rats given the extract of Magnoliae Officinalis Cortex, the extract of Magnoliae Officinalis Cortex compatibility with Frutus auranti, cisapride were (69.3±13.7)%, (60.7±12.9)% and (70.1±12.9)% respectively; plasma motilin were (85.4±11.2), (93.1±10.4) and (86.3±12.4)nmol ·L^-1 accordingly. Gastric residual rate was lower (P<0.05) and plasma motilin was higher(P<0.05) for the decoction duration of 90 min compared with those of duration 60 min or 30 min. Gastric residual rate for the decoction duration 90, 60,30 min were (47.7±12.8)%, (59.8±12.1)% and (62.7±11.8)% respectively;plasma motilin were (102.3±10.6), (92.9±10.1) and (89.6±11.2) nmol ·L^-1 respectively. Conclusion: The extract of Magnoliae Officinalis Cortex, Magnoliae Officinalis Cortex compatibility with Aurantii Frutus Immaturus can effectively improve the gastric motility disorder in rats, and Magnoliae Officinalis Cortex compatibility with Aurantii Frutus Immaturus is better than Magnoliae Officinalis Cortex alone. The recommended decoction duration is 90 min.