妇科千金片对急性盆腔炎模型大鼠外周血细胞及盆腔组织病理改变的影响

目的:观察妇科千金片对急性盆腔炎大鼠外周血血细胞及子宫、卵巢、输卵管组织病理改变的影响,探讨该药治疗盆腔炎的作用机制。方法:采用混合菌(大肠杆菌、解脲支原体和金黄色葡萄球菌)接种法建立急性盆腔炎大鼠模型,造模成功后随机分别为空白组、假手术组、模型组、妇科千金片低、中、高剂量组(0.21,0.63,1.89 g.kg-1)、中药对照组(妇炎康1.23 g.kg-1)及西药对照组(罗红霉素82.74 mg.kg-1)。药物治疗21 d后取外周血及盆腔组织,生化分析仪分析血液中常规成分变化并观察大鼠盆腔组织的病理改变。结果:①与空白组和假手术组相比,模型组白细胞总数、中性粒细胞比例升高而淋巴细胞比例下降,且盆腔组织出现明显炎症改变。②与模型组相比,各治疗组白细胞总数、中性粒细胞比例下降而淋巴细胞比例升高,且盆腔组织病变明显改善。③与西药对照组及中药对照组相比,妇科千金片各治疗组白细胞数无统计学差异,但对盆腔病变改善情况优于中药对照组。④血红蛋白含量、血小板、嗜酸性粒细胞及嗜碱性粒细胞各组间比较无差异。结论:妇科千金片对大鼠免疫细胞有显著影响并对盆腔组织有一定保护作用,其抗炎作用的机制可能是通过提高机体免疫力从而抑杀进入体内病原菌而实现。

Influence of Fuke Qianjin Tablets on Blood Cells and Histopathological Manifestations of Acute Pelvic Inflammation in Rats

Objective: To investigate the effects of Fuke Qianjin tablets on the blood cells and histopathological manifestationsof acute pelvic inflammatory in rats. Method: The model of acute pelvic inflammation was established by injecting three kinds of bacteria (Escherichia coli,Mycoplasma urealyticum and Staphyloccocus aureus Rosenbach) into the uterus. The rats were randomLy divided into 8 groups and the patterns of blood cells were tested after 21 days of treatment. Result: Compared with normal group and shaml group, the white blood cells and proportions of neutrophils in model group were significantly lowered but proportions of lymphocytes increased which indicated a significant pelvic inflammation. Compared with model group, the white blood cells and proportions of neutrophils in treat groups were significantly increased but proportions of lymphocytes lowered. Conclusion: Fuke Qianjin tablets can influence the bloods cells and immunocytes to protect pelvic tissues.