D101大孔树脂吸附芒果苷的影响因素

目的:筛选可有效吸附芒果的树脂,优化影响芒果苷在D101大孔树脂上的吸附和洗脱条件。方法:采用静态吸附法筛选对芒果苷有良好吸附作用的树脂;采用正交试验方法,研究药液pH、浓度、温度、时间对D101大孔树脂吸附芒果苷的影响;研究不同体积分数乙醇对芒果苷的解析作用。结果:供试的41种树脂中,大孔树脂D101和AB-8最好;D101对芒果苷吸附的影响因素,最大的为pH和吸附时间,吸附温度次之,溶液浓度影响最小;70%乙醇水溶液的洗脱效果最好。结论:采用D101吸附芒果苷,最佳条件为pH 2水溶液,芒果苷浓度2.841×10-2 mol.mL-1,常温吸附,时间5 min以上;用70%乙醇水溶液洗脱。

Operation Conditions Affecting Macroporous Resin D101 to Adsorb Mangiferin

Objective: To select one or more macroporous resins that can effectively adsorb mangiferin, and then optimize the operation conditions. Method: Static adsorption test was employed to select target resins, and orthogonal test was used to optimize the operation conditions. Result: Macroporous resin D₁₀₁ and AB-8 were the best in 41 tested resins. The operation conditions, arranged from high to low, were pH, time, temperature and concentration. 70% alcohol aqueous could effectively desorb mangiferin from resin D101. Conclusion: The optimal conditions for macroporous resin D₁₀₁ to adsorb mangiferin are: pH 2 aqueous solution with a mangiferin concentration of 2.841×10^-2 mol ·L^-1, adsorption time is 5 minutes, operating at room temperature; 70% alcohol aqueous was used as desorption solution.