抗柯萨奇B病毒性心肌炎胶囊总黄酮对小鼠 急性阿霉素心肌损伤的保护作用

目的:观察抗柯萨奇B病毒性心肌炎胶囊(K-CoxB-JN)总黄酮对阿霉素所致小鼠急性心肌损伤的作用,并探讨其可能作用机制。方法:选取正常昆明种小鼠175只,随机分为7组,即K-CoxB-JN黄酮高、中、低剂量组(0.8,0.4,0.2 g.kg-1),模型组,心血康组,正常组,原方水煎组,每组25只,每天ig 1次,1周后ip阿霉素20 mg.kg-1建立小鼠急性心肌损伤模型,24 h后眼球取血测定血清心肌酶水平、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,测定心脾指数。结果:K-CoxB-JN总黄酮能显著降低小鼠心肌酶活性,黄酮中剂量组乳酸脱氢酶(LDH)水平有所下降,α-羟丁酸脱氢酶(HBDH)水平降低明显(P<0.01);黄酮高、中剂量组心脏指数明显降低(P<0.01),黄酮中剂量组脾指数有显著性差异(P<0.05)。黄酮高、中剂量组SOD活力明显升高(P<0.01),黄酮中剂量组MDA含量有显著性降低(p<0.01),以黄酮中剂量效果最为明显。结论:K-CoxB-JN总黄酮对小鼠急性心肌损伤有保护作用,其作用机制可能与抗氧化、清除氧自由基有关。

Protection of Total Flavonoids in K-CoxB-JN Against Acute Myocardial Injury Caused by Adriamycin in Mice

Objective: to observe protection of total flavonoids in anti-coxsackie B complex capsule(K-CoxB-JN) against acute myocardial injury caused by adriamycin in mice and to discuss the feasible mechanism. Method: One hundred and seventy five Kunming mice were selected and diveded into 7 groups, including high dose group of flavonoid (0.8 g·kg^-1), middle dose group of flavonoid(0.4 g·kg^-1), low dose group of flavonoid, model group(0.2 g·kg^-1), Xinxuekang group, blank group and original formula group., there 25 mice in each group are taken. After a week, model of acute myocardial injury was established by adriamycin injection of 20 mg·kg^-1. Blood was collected after 24 h of model building, then determination of myocardial enzyme like SOD and MDA were performed as well as indexes of heart and spleen. Result: Activity of myocardial enzymes was decreased drastically with total flavonoids in K-CoxB-JN, as well as indexes of heart and spleen, especially middle dose group of flavonoid. Conlusion: Total flavonoids in K-CoxB-JN do prectect mice against acute myocardial injury. The possible meachanism may be related to antioxidation and scavenging of oxygen free radicals.