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| 响应面法优化仙鹤草总黄酮的超声提取工艺 | |
| 引用本文： | 蒋志华,俞力超,冯子旺,李峰,徐秀泉. 响应面法优化仙鹤草总黄酮的超声提取工艺[J]. 中国实验方剂学杂志, 2011, 17(20): 17-20 |
| 作者姓名： | 蒋志华 俞力超 冯子旺 李峰 徐秀泉 |
| 作者单位： | 1. 江苏大学附属医院,江苏镇江,212001 2. 江苏大学临床医学院,江苏镇江,212013 3. 江苏大学药学院,江苏镇江,212013 |
| 基金项目： | 镇江市社会发展项目(SH2010033) |
| 摘 要： | 目的:优化仙鹤草总黄酮的最佳超声提取工艺。方法:以总黄酮得率为指标,采用响应面设计方法对影响总黄酮得率的乙醇体积分数、超声温度及料液比进行优化。结果:各因素对总黄酮提取率的影响大小依次为料液比-超声温度-乙醇体积分数。最佳工艺条件为72%乙醇,料液比1∶25,60℃下超声提取20 min。在此最佳条件下,总黄酮得率为9.02 mg.g-1,试验结果与模型预测值相符。结论:利用超声提取仙鹤草总黄酮工艺稳定可靠。 |
| 关 键 词： | 仙鹤草 总黄酮 超声提取 响应面设计 |
| 收稿时间： | 2011-06-15 |
Optimization of Ultrasound Extraction Process of Total Flavonoids from Agrimonia pilosa by Response Surface Methodology |
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| JIANG Zhi-hu,YU Li-chao,FENG Zi-wang,LI Feng and XU Xiu-quan. Optimization of Ultrasound Extraction Process of Total Flavonoids from Agrimonia pilosa by Response Surface Methodology[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(20): 17-20 | |
| Authors: | JIANG Zhi-hu YU Li-chao FENG Zi-wang LI Feng XU Xiu-quan |
| Affiliation: | Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China;Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China;School of Clinical Medical, Jiangsu University, Zhenjiang 212013, China;Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China;School of Pharmacy, Jiangsu University, Zhenjiang 212013, China |
| Abstract: | Objective: To optimize optimum ultrasound extraction process of total flavonoids from Agrimania pilosa.Method: With yield of total flavonoids as index,ethanol concentration,ultrasound temperature and material-liquid ratio were optimized by response surface methodology(RSM).Result: Effect factors on extraction rate of total flavonoids with the order of follows: material-liquid ratio,ultrasound temperature,ethanol concentration.The optimal extraction conditions were ethanol concentration was 72%,material-liqu... |
| Keywords: | Agrimania pilosa total flavonoids ultrasound extraction response surface methodology |
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