高效液相色谱法测定人血浆中苦参碱的浓度

目的建立高效液相色谱（HPLC）法测定人血浆中苦参碱的浓度。方法以0．02mol／L磷酸二氢钾溶液（含0．4％三乙胺，以磷酸调节pH值至6.0±0．1）-乙腈（88：12）为流动相，尼扎替丁为内标，血浆样品经二氯甲烷萃取后采用HPLC法测定。结果苦参碱质量浓度的线性范围是0．102～20．4μg／mL，平均相对回收率为93．3％～108，6％，日内和日间精密度RSD均小于10％，苦参碱的最低检测限为25ng／mL。结论该方法快速、准确、灵敏，可用于苦参碱的药代动力学研究。

Determination of Matrine in Human Plasma by HPLC

Objective To establish a HPLC method for detecting the concentration of matrine in human plasm. Methods The mobile phase consisted of 0. 02 mol/L potassium dihydrogen phosphate solution（containing 0.4% triethylamine,adjusted to pH 6. 0 ±0. 1 with phosphoric acid）- acetonitrile （88：12）was used. Nizatidine was used as the internal standard. The plasm samples were pretreated by dichloromethane, then determined by HPLC. Results The range of linear was 0. 102-20. 4 μg/mL. Both the intra- day and inter-day RSD were less than 10%. The recovery rate was 93.3% -108.6%. The minimum detective limit was 25 ng/mL. Conclusion The HPLC method is accurate,stable and sensitive. It can be applied to clinical pharmacokinetics study.