粘着斑激酶在大肠癌中表达水平与侵袭、转移的相关性研究

目的研究粘着斑激酶 (FAK)表达与大肠癌发生、生物学行为和预后的关系。方法应用免疫组织化学S -P方法检测 10例正常肠粘膜、16例肠腺瘤伴不典型增生及 10 8例大肠癌手术标本中FAK的表达。结果正常肠粘膜表达均阴性、肠腺瘤伴不典型增生表达阳性率为 12 .5 0 % ,大肠癌表达阳性率为 75 .93% (82 / 10 8)。其中无区域淋巴结转移者阳性率为 48.84% ,有区域淋巴结转移者阳性率为 93.85 % (P <0 .0 1) ,FAK表达水平与大肠癌浸润深度和Dukes'分期呈正相关 (均 P<0 .0 1)。FAK阳性者术后 3年、5年生存率分别为 49.74%、44 .30 %。而阴性者为 10 0 %、88.5 4%。二组患者术后 3年、5年生存率差别均有显著性意义 (均P <0 .0 1)。结论大肠癌由于FAK表达增加 ,加速恶性细胞的增殖、浸润和转移。故检测大肠活检粘膜及癌组织中FAK表达状况 ,有助于进一步了解大肠粘膜癌变倾向及大肠癌生物学行为 ,有利于对患者预后的判断。

Study on Correlation Between FAK Expression Levels and Invasion/Metastasis of Colorectal Carcinoma

ObjectiveTo study the correlations between focal adhesion kinase(FAK) expression and carcinogenesis of colorectal mucosa, biological behavior and prognosis in colo rectal carcinoma. MethodUsing anti-monoclonal antibody, expression of FAK was examined in specimens of colorectal mucosa from 10 healthy subjects, 16 cases of intestinal adenoma associated with atypical colorectal mucosa dysplasia and 108 cases of resected colorectal carcinoma(CRC) by immunohistochemical(S-P)method. Results Normal colorectal mucosa was negative for FAK expression, but a relatively low positive expression rate (12.5%)was found in cases with dysplasia. Positive expression rate of FAK in 108 cases with CRC was 75.93%(82/108). CRCs without regional lymph nodal metastasis showed significantly lower positive rate (48.84%) than those with lymph nodal involvement(93.85%)(P<0.01).Expression levels of FAK was positively correlated with the invasive depth of CRC and progression of Dukes stage(P<0.01;P<0.01). The survival rate in patients with positive FAK expression 3 and 5 years after operation was 49.71% and 44.30% respectively, when compared with that in those of negative FAK expression(100% and 88.54% respectively). Significant difference existed between 3 and 5 year-survival rate of two groups(P<0.01). ConclusionsThese data indicate that increased expression of FAK can accelerate the proliferation, invasion and metastasis of malignant cells in CRC. Therefore, detection of FAK expression in biopsied colorectal mucosa and cancer tissues would further help to understand the carcinogenic tendency of colorectal mucosa and biological behavior of CRC as well as to make judgment of patient’s prognosis.