| 不同直径神经干细胞球慢速低温冻存的研究 |
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| 引用本文： | 马学虎,侯颖,石颖,张蕾,范文霞,葛丹,刘天庆,崔占峰.不同直径神经干细胞球慢速低温冻存的研究[J].中国生物医学工程学报,2009,28(6). |
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| 作者姓名： | 马学虎 侯颖 石颖 张蕾 范文霞 葛丹 刘天庆 崔占峰 |
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| 作者单位： | 1. 大连理工大学大连市干细胞与组织工程研究中心,大连,116024
2. 大连理工大学大连市干细胞与组织工程研究中心,大连,116024;牛津大学工程科学系,英国牛津OX1,3PJ |
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| 基金项目： | 国家自然科学基金项目，大连市科技计划资助项目 |
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| 摘 要： | 为提高神经干细胞冻存复苏后的效果及存活率,对不同尺寸与状态的神经干细胞球进行了在不同浓度DMSO(二甲基亚砜)下的慢速冻存比较.首先用CCK-8试剂盒测定神经干细胞的生长曲线,然后对处于对数生长期的3类神经干细胞(单细胞悬液,直径30～50 μm球,直径80～100 μm球)分别进行了7个不同浓度(3%,5%,7%,8%,10%,15%,20%)DMSO的冻存实验比较,并对冻后复苏的细胞进行活性检测和诱导分化.结果表明, 直径80～100 μm的神经干细胞球,DMSO浓度8%,复苏后细胞活率82.9%,且仍具有多项分化潜能.神经干细胞间的亲密联系和刻痕信号与直径尺寸共同作用,影响着神经干细胞的冻存复苏效果.
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| 关 键 词： | 神经干细胞 细胞球直径 慢速冻存 |
Slow Freezing Cryopreservation of Neural Stem Cell Spheres with Different Diameter |
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| MA Xue-Hu,HOU Ying,SHI Ying,ZHANG Lei,FAN Wen-Xia,GE Dan,LIU Tian-Qing,CUI Zhan-Feng.Slow Freezing Cryopreservation of Neural Stem Cell Spheres with Different Diameter[J].Chinese Journal of Biomedical Engineering,2009,28(6). |
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| Authors: | MA Xue-Hu HOU Ying SHI Ying ZHANG Lei FAN Wen-Xia GE Dan LIU Tian-Qing CUI Zhan-Feng |
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| Abstract: | This study aims to improve the survival rate of the cryopreserved neural stem cell (NSC) spheres with different diameters by slow freezing with different DMSO concentrations.After measuring the growth curve by CCK-8 kit, three sorts of NSCs states in log phase (single cell suspension, NSC spheres with diameters of 30～50 μm and 80～100 μm) were respectively cryopreserved by seven concentrations of DMSO (3%, 5%, 7%, 8%, 10%, 15% and 20%) with the freezing rate of 1℃/min.Then the post-thaw NSCs were tested for the survival rate and the differentiation ability.The best result was that NSC sphere with diameters of 80～100 μm and DMSO concentration of 8% achieved the survival rate of 82.89% while remaining the multi-differentiation potentiality.The subtle cooperation of NSCs and sphere diameters affect the survival rate together. |
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| Keywords: | DMSO NSCs sphere diameter DMSO slow freezing cryopreservation |
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