A new method for the assay of aminoacylase: elaboration of a fixed-incubation method for routine measurements. |
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Authors: | K Lorentz J Voss B Flatter |
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Affiliation: | I. Medizinische Klinik der Medizinischen Hochschule Lübeck, 24 Lübeck and Institut für Organische Chemie und Biochemie der Universität Hamburg, 2 HamburgG.F.R. |
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Abstract: | A simple method suitable for routine determinations of aminoacylase (EC 3.5.1.14) in serum and tissue homogenates is described. It is based on the formation of red charge-transfer complexes from p-benzoquinone and amino acids liberated by cleavage of acylamino acids. Optimal substrates are trifluoroacetyl-L-methionine and chloroacetyl-L-methionine, the first being rapidly hydrolyzed by pancreatic tissue, the latter by liver and kidney homogenates. Sera preferentially split the chloroacetyl compound. Optimal conditions for the assay of serum activity are: 18-20 mm9l/l substrate concentration, 50 mmol/l phosphate buffer pH 6.0, no additives. |
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Keywords: | Correspondence to: Professor Dr K. Lorentz I. Medizinische Klinik der Medizinischen Hochschule D-24 Lübeck Kronsforder Allee 71/73 G.F.R.. |
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