| 雄激素受体反义RNA抑制前列腺癌细胞LNCaP致瘤性的研究 |
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| 引用本文： | 江军,王洛夫,方玉华,靳风烁,靳文生.雄激素受体反义RNA抑制前列腺癌细胞LNCaP致瘤性的研究[J].第三军医大学学报,2003,25(15):1336-1338. |
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| 作者姓名： | 江军 王洛夫 方玉华 靳风烁 靳文生 |
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| 作者单位： | 第三军医大学附属大坪医院野战外科研究所泌尿外科,重庆,400042 |
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| 基金项目： | 国家自然科学基金资助项目 ( 39770 74 0 ) |
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| 摘 要： | 目的 观察雄激素受体 (Androgenreceptor ,AR)反义RNA对前列腺癌细胞LNCaP致瘤性的影响。方法 分别用细胞平板克隆形成试验及裸鼠接种实验观察前列腺癌细胞LNCaP、LNCaPNeo和LNCaPas AR 细胞致瘤性的变化。结果 LNCaPas AR细胞的克隆形成效率显著低于LNCaPNeo细胞 (P <0 .0 1)。LNCaPas AR细胞在雄性裸鼠体内形成的瘤体体积明显小于LNCaPNeo细胞并且形成瘤体的时间延长。结论 雄激素受体反义RNA可抑制前列腺癌细胞LNCaP成瘤能力。
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| 关 键 词： | 雄激素受体 反义RNA 前列腺癌 |
| 文章编号： | 1000-5404(2003)15-1336-03 |
| 修稿时间： | 2003年3月6日 |
Inhibitive effect of androgen receptor antisense RNA on tumorigenesis of human prostate cancer cell LNCaP |
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| JIANG Jun,WANG Luo fu,FANG Yu hua,JIN Feng shuo,JIN Wen sheng.Inhibitive effect of androgen receptor antisense RNA on tumorigenesis of human prostate cancer cell LNCaP[J].Acta Academiae Medicinae Militaris Tertiae,2003,25(15):1336-1338. |
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| Authors: | JIANG Jun WANG Luo fu FANG Yu hua JIN Feng shuo JIN Wen sheng |
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| Abstract: | Objective To investigate the effects of AR antisense RNA on tumorigenesis of LNCaP cells. Methods Tumor formation abilities of LNCaP, LNCaP Neo and LNCaP as AR cells were determined by plate cloning formation efficiency in vitro and by injection of the cells into intact male nude mice. Results Cloning formation efficiency of LNCaP as AR cells in vitro was significantly lower ( P <0.05) than that of LNCaP and LNCaP Neo . LNCaP as AR tumor formation in the nude mice in vivo was inhibited and time for tumor formation was prolonged as compared with that of LNCaP and/or LNCaP Neo . There was no difference between LNCaP and LNCaP Neo ( P >0.05). Conclusion Antisense AR RNA can inhibit tumor formation of prostate carcinoma cell LNCaP. |
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| Keywords: | androgen receptor antisense RNA prostate carcinoma cell |
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