聚磷酸钙纤维/磷酸钙骨水泥/微小颗粒骨修复骨缺损的实验研究

目的探讨聚磷酸钙纤维(calcium polyphosphate fibers,CPPF)、磷酸钙骨水泥(calcium phosphate cement,CPC)、自体微小颗粒骨复合材料修复骨缺损的能力。方法选用新西兰大白兔72只,双侧桡骨制成1.5cm骨缺损模型,随机分成6组分别植入。A组:CPPF/CPC/微小颗粒骨复合材料;B组:CPC/微小颗粒骨复合材料;C组:单纯微小颗粒骨;D组:CPPF/CPC复合材料;E组:单纯CPC;F组:空白对照组,不植入任何物质。在2、4、8、12周各时相点,分别进行大体观察、X线照片、组织学切片、扫描电镜观察及力学测试。结果A组在12周可使骨缺损修复,在骨缺损修复各时期,其成骨速度及成骨量均好于其他各组。结论CPPF/CPC/微小颗粒骨复合材料有良好的成骨能力、力学特性和生物相容性,有望成为骨组织工程中修复骨缺损的理想材料。

Repair of Bone Defect with Composite of Calcium Polyphosphate Fibers,Calcium Polyphosphate and Micromorselized Bone

Objective To evaluate the ability of the composite of composite of calcium polyphosphate fibers(CPPF)/calcium polyphosphate(CPC) /micromorselized bone in repair of bone defects.Methods The animal models of bilateral radius bone defect of 15 mm length created by surgery in the New Zealand white rabbits were implanted respectively with CPPF/CPC/micromorselized bone,CPC/micromorselized bone,micromorselized bone,CPPF/CPC and CPC.The bone defects with no implant were used as blank control group.The effect was observed by gross,X-ray,histopathological,scanning electron microscopic,and dynamic examinations 2,4,8 and 12 weeks after operation.Results The group with CPPF/CPC/micromorselized bone exhibited the strongest defect-repairing ability,and obvious healing of defects could be seen 12 week postoperatively.At every stage,the group with CPPF/CPC/micromorselized bone did a better job than the other groups in term of quantity and quality of new bone growth.Conclusion CPPF/CPC/micromorselized bone has good biocompatibility,dynamic characteristics and osteogenic ability and may be one of ideal scaffolds in repair of bone defects in bone tissue engineering.