| 磷酸肌酸对大鼠心肌缺氧、复氧心肌线粒体肌酸激酶肌膜型亚基mRNA表达水平的影响 |
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| 引用本文： | 李芳,李辉,朱世军,王孝铭,于康振.磷酸肌酸对大鼠心肌缺氧、复氧心肌线粒体肌酸激酶肌膜型亚基mRNA表达水平的影响[J].中国病理生理杂志,2000,16(2):143-145. |
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| 作者姓名： | 李芳 李辉 朱世军 王孝铭 于康振 |
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| 作者单位： | 1. 解放军211医院肾内科，黑龙江 哈尔滨 150086；2. 哈尔滨医科大学病理生理教研室，黑龙江 哈尔滨 150086；3. 兽医生物技术国家重点实验室，黑龙江 哈尔滨 150001 |
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| 基金项目： | 黑龙江省自然科学基金资助! (D0 96 13) |
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| 摘 要： | 目的和方法：以离体心脏灌流法制备大鼠心肌缺氧、复氧模型，提取大鼠心矶ｍＲＮＡ并标记心肌线粒体肌酸激酶肌膜型亚基基因探针，进行Ｎｏｒｔｈｅｒｎ杂交，观察不同缺氧、复氧时ｓＭｉＭｉＣＫｍＲＮＡ的变化及磷酸肌物保护作用。结果：随着缺氧时间的延长，ｓＭｉＭｉ－ＣＫ的ｍＲＮＡ逐渐减少，复氧后ｓＭｉＭｉ－ＣＫ进一步减少，ＰＣｒ能显著改善ｓＭｉＭｉ－ＣＫ的表达量。结论：ＰＣｒ对缺氧、复氧的心肌具有明显的保护作用
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| 关 键 词： | 大鼠 心肌 低氧 RNA 信使 磷酸肌酸 |
| 收稿时间： | 1998-9-10 |
The influence of phosphocreatine on expression of mitochondrial sarcomeric creatine kinase mRNA of myocardium ischemia and reperfusion in rats |
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| LI Fang,LI Hui,ZHU Shi-jun,WANG Xiao-ming,YU Kang-zhen.The influence of phosphocreatine on expression of mitochondrial sarcomeric creatine kinase mRNA of myocardium ischemia and reperfusion in rats[J].Chinese Journal of Pathophysiology,2000,16(2):143-145. |
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| Authors: | LI Fang LI Hui ZHU Shi-jun WANG Xiao-ming YU Kang-zhen |
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| Institution: | 1. Department of Pathophysiology, Harbin Medical University, Harbin 150086; 2. Harbin Veterinary Research Institute, Harbin 150001, China |
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| Abstract: | AIM and METHODS: Rat myocardial mRNA was extracted and mitochondrial sarcomeric creatine kinase (sMiMi-CK) was used as labled probed in vitro myocardial ischemia/reperfusion rats. Northern blot was performed to determine the changes of sMiMi-CK mRNA in different periods of ischemia/reperfusion and the protective effect of phosphocreatine (PCr).RESULTS:During early ischemial (10 min) mRNA was decreased about 18 24%. After reperfusion mRNA decreased again. PCr effectively prevented the decrease of mRNA expression of sMiMi-CK. CONCLUSION:The results indicated that PCr may possess protective effect on anoxic and reoxygenated myocardium. |
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| Keywords: | Rats Myocardium Anoxia RNA messenger Phosphocreatine |
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