| STAT3基因沉默抑制人白血病细胞体外生长的实验研究 |
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| 引用本文： | 马玲娣,周 民,蒋丽佳,岑 玲,范 静,夏 蕾.STAT3基因沉默抑制人白血病细胞体外生长的实验研究[J].南京医科大学学报,2011(2):149-155. |
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| 作者姓名： | 马玲娣 周 民 蒋丽佳 岑 玲 范 静 夏 蕾 |
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| 作者单位： | 南京医科大学附属常州第二人民医院中心实验室;南京医科大学附属常州第二人民医院中心血液科; |
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| 基金项目： | 江苏常州卫生创新人才基金 |
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| 摘 要： | 目的:构建人STAT3 shRNA(short hairpin RNA)慢病毒载体,研究慢病毒介导的STAT3 SiRNA(small interferenceRNA)对人慢性粒细胞白血病K562细胞体外生长特性的影响。方法:针对STAT3基因RNAi有效靶点构建STAT3 shRNA慢病毒载体,293T细胞内包装产生STAT3 SiRNA慢病毒,感染K562细胞后RT-PCR和Western-blot方法验证STAT3基因的敲减效率;细胞生长曲线和MTT法观察STAT3敲减后K562细胞体外生长和增殖情况;流式细胞术和Annexin V-FITC/PI法检测细胞周期改变和早期凋亡。结果:成功得到STAT3 SiRNA慢病毒,RT-PCR和Western-blot实验证实,针对其中两个靶点的STAT3 SiRNA对STAT3基因的敲减效率均在70%以上(P<0.05)。STAT3敲减后,K562细胞生长变慢,细胞增殖活性降低40%(P<0.05);细胞阻滞于G1→S期,并出现早期凋亡。结论:STAT3 SiRNA慢病毒可有效抑制K562细胞内STAT3基因表达,使细胞生长增殖受抑,细胞周期阻滞,细胞出现早期凋...
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| 关 键 词： | STAT3基因 RNA干扰 慢病毒 K562细胞 凋亡 |
| 收稿时间： | 8/6/2010 12:00:00 AM |
Effect of STAT3 gene silencing on growth of chronic myelogenous leukemia K562 cells |
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| MA Ling-di,ZHOU Min,JIANG Li-ji,CEN Ling,FAN Jing and XIA Lei.Effect of STAT3 gene silencing on growth of chronic myelogenous leukemia K562 cells[J].Acta Universitatis Medicinalis Nanjing,2011(2):149-155. |
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| Authors: | MA Ling-di ZHOU Min JIANG Li-ji CEN Ling FAN Jing and XIA Lei |
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| Institution: | MA Ling-di*,ZHOU Min1,JIANG Li-jia,CEN Ling1,FAN Jing,XIA Lei(Center Laboratory,1Department of Hematology,Second Changzhou People's Hospital Affiliated to NJMU,Changzhou 213003,China) |
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| Abstract: | Objective: To construct the STAT3 shRNA(short hairpin RNA) lentivirus and investigate the effects of STAT3 SiRNA(small interference RNA)on the biological property of human chronic myelogenous leukemia(CML) K562 cells.Methods:The STAT3 shRNA lentiviral vector was constructed according to the effective target sequences for RNAi of STAT3 gene.The STAT3 SiRNA lentivirus were produced by transfecting into packaging cell line 293T.After transfected into K562 cells,the knockdown of STAT3 gene were determined by RT... |
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| Keywords: | STAT3 RNA interference lentivirirus K562 cell line apoptosis |
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