| 雷公藤内酯醇对人绒毛膜癌细胞株JAR增殖凋亡及凋亡调控蛋白BCL-2/BAX表达的影响 |
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| 引用本文： | 梁向华,黄骁昊. 雷公藤内酯醇对人绒毛膜癌细胞株JAR增殖凋亡及凋亡调控蛋白BCL-2/BAX表达的影响[J]. 南京医科大学学报(自然科学版), 2011, 0(2): 166-169 |
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| 作者姓名： | 梁向华 黄骁昊 |
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| 作者单位： | 启东市人民医院妇产科;南京医科大学第一附属医院妇产科; |
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| 摘 要： | 目的:探讨雷公藤内酯醇(triptolide,TP)对人绒癌细胞株JAR增殖、凋亡及凋亡调控蛋白BCL-2/BAX表达的影响。方法:以体外培养的人绒癌细胞株JAR为研究对象,MTT法检测TP对细胞增殖的抑制情况;TUNEL法观察TP作用于细胞后引起细胞凋亡情况,PCR和Western blot检测TP对凋亡调控蛋白BCL-2/BAX mRNA和蛋白表达的影响。结果:随着作用浓度的增加及时间延长,TP能明显抑制JAR细胞增殖并诱导其凋亡(P<0.05);并能下调凋亡抑制蛋白BCL-2的mRNA和蛋白的表达,上调凋亡促进蛋白BAX的mRNA和蛋白表达(P均<0.05)。结论:雷公藤内酯醇在体外能抑制人绒癌细胞株JAR增殖,诱导其凋亡;凋亡调控蛋白BCL-2/BAX表达水平的变化可能是TP诱导JAR细胞凋亡的重要机制。
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| 关 键 词： | 雷公藤内酯醇 绒癌 凋亡 增殖 BCL-2/BAX |
| 收稿时间： | 2010-07-22 |
The effect of triptolide on the proliferation, apoptosis and the expression of BCL-2/BAX of human choriocarcinoma cell line JAR in vitro |
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| LIANG Xiang-hua and HUANG Xiao-hao. The effect of triptolide on the proliferation, apoptosis and the expression of BCL-2/BAX of human choriocarcinoma cell line JAR in vitro[J]. Acta Universitatis Medicinalis Nanjing, 2011, 0(2): 166-169 |
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| Authors: | LIANG Xiang-hua and HUANG Xiao-hao |
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| Affiliation: | LIANG Xiang-hua,HUANG Xiao-hao1(Department of Obstetrics and Gynecology,the People's Hospital of Qidong,Qidong 226200,1Department of Gynaecology and Obstetrics,the First Affiliated Hospital of NJMU,Nanjing 210029,China) |
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| Abstract: | Objective: To investigate the effect of triptolide(TP) on the proliferation,apoptosis and the expression of BCL-2/BAX of human choriocarcinoma cell line JAR in vitro.Methods:Human choriocarcinoma cell line JAR was cultured.After the treatment with triptolide,MTT assay was used to detect the inhibitive effect of triptolide on cell proliferation;TUNEL assay was exploited to determine the level of cell apoptosis;Western blot was used to observe BCL-2/BAX expression.Results: TP could inhibit the proliferation o... |
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| Keywords: | triptolide choriocarcinoma proliferation apoptosis BCL-2/BAX |
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