RP-HPLC法测定葫芦素B月桂酸酯纳米乳剂中药物的含量

目的建立测定葫芦素B月桂酸酯纳米乳剂中药物含量的方法。方法采用Diamonsil ODS柱(钻石)C18柱(200 mm×4.6 mm,5μm),流动相为甲醇-水(体积比为95∶5),流速1.0 mL.min-1,检测波长228 nm,柱温为室温。结果在本试验条件下,葫芦素B月桂酸酯与辅料及有关物质分离度均符合要求,其质量浓度在15~150 mg.L-1内与峰面积呈良好线性关系(r=0.999 9,n=5),方法平均回收率为99.98%,RSD为0.1%(n=9)。结论该方法简单、快捷,可用于葫芦素B月桂酸酯纳米乳剂中药物含量的测定。

Determination of cucurbitacin B laurate in nanoemulsions by RP-HPLC

Objective To establish an RP-HPLC method for the determination of cucurbitacin B laurate in nanoemulsions. Methods The separation was performed with a Diamonsil ODS column (200 mm×4.6 mm, 5 μm) at room temperature, and the mobile phase was consisted of methanol-water (V︰V=95︰5), the flow rate was 1.0 mL·min^-1. Cucurbitacin B laurate was detected at 228 nm. Results There was a good linear relationship between A and concentration over the range of 15-150 mg·L^-1 (r=0.999 9, n=5). The average recovery was 99.98%. The RSD was 0.1%(n=9). Conclusion This method is applicable for the determination of cucurbitacin B laurate in nanoemulsions.