| 日本血吸虫未知功能基因SJFCE3549的克隆及生物信息学分析 |
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| 引用本文： | 胡君健,贺佩,王媛园,孙成松,喻鑫玲. 日本血吸虫未知功能基因SJFCE3549的克隆及生物信息学分析[J]. 实用预防医学, 2011, 18(6): 978-981 |
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| 作者姓名： | 胡君健 贺佩 王媛园 孙成松 喻鑫玲 |
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| 作者单位： | 湖南省血吸虫病防治所,湖南岳阳,414000 |
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| 基金项目： | 湖南省卫生厅基金资助项目B2009102 |
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| 摘 要： | 目的克隆与日本血吸虫未知功能的新基因SJFCE3549,构建其真核表达载体,并应用生物信息学初步探讨其功能。方法应用PCR技术从日本血吸虫成虫cDNA文库中扩增SJFCE3549基因全长ORF,将其亚克隆到真核表达载体pCMV-Tag2A中,通过PCR、限制性酶切分析及测序进行鉴定。应用生物信息学初步分析其细胞定位、蛋白序列、结构域及功能。结果获得日本血吸虫未知功能基因的克隆SJFCE3549,序列分析结果提示该cDNA序列含有一个453 bp的完整阅读框序列,编码150个氨基酸,其编码蛋白的理论分子量为16.67 kDa,等电点为6.28。二级结构分析预测提示其具有一定抗原性。结论成功地克隆了日本血吸虫基因SJFCE3549全长ORF,构建了其pCMV-Tag2A真核表达载体,为进一步研究其结构与功能创造了条件。
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| 关 键 词： | 日本血吸虫 基因克隆 生物信息学 |
Molecular Cloning and Bioinformatic Analysis of a Novel Gene of SJFCE3549 Schistosoma japonicum |
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| HU Jun-jian,HE Pei,WANG Yuan-yuan,et al.. Molecular Cloning and Bioinformatic Analysis of a Novel Gene of SJFCE3549 Schistosoma japonicum[J]. Practical Preventive Medicine, 2011, 18(6): 978-981 |
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| Authors: | HU Jun-jian HE Pei WANG Yuan-yuan et al. |
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| Affiliation: | HU Jun-jian,HE Pei,WANG Yuan-yuan,et al.(Hunan Institute of Parasitic Diseases,Yueyang 414000,Hunan,China) |
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| Abstract: | Objective To clone Schistosoma japonicum SJFCE3549,a novel gene,to construct its recombinant eukaryofic expression vector,and to preliminarily explore its function and roles through bioinformatic methods.Methods The full-length ORF of Schistosoma japonicum SJFCE3549 was amplified by PCR from cDNA library of Schistosoma japonicum.Recombinant eukaryofic expression vector(pCMV-Tag2A-SJFCE3549) was then constructed by sub-cloning technique and confirmed by restriction enzyme digestion analysis and sequencing.Bi... |
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| Keywords: | Schistosoma japonicum Gene cloning Bioinformatics |
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