Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis |
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| Authors: | Rigouts Leen Nolasco Oscar de Rijk Pim Nduwamahoro Elie Van Deun Armand Ramsay Andy Arevalo Jorge Portaels Françoise |
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| Affiliation: | Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerpen, Belgium. lrigouts@itg.be |
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| Abstract: | New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497. |
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