Genetic characterization of a rare bovine-like human VP4 mono-reassortant G6P[8] rotavirus strain detected from an infant in Bangladesh |
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| Affiliation: | 1. Programa de Pós Graduação em Biologia Parasitária da Amazônia, Universidade do Estado do Pará, Instituto Evandro Chagas, Brazil;2. Programa de Pós Graduação em Virologia do Instituto Evandro Chagas, Brazil;3. Programa Institucional de Bolsas de Iniciação Científica, CNPq-Instituto Evandro Chagas, Brazil;4. Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Brazil;5. Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium;1. Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa;2. Centro de Investigação em Saúde de Manhiça (CISM), Manhiça, Mozambique;3. Institute of Hygiene and Tropical Medicine, Lisbon, Portugal;4. Next Generation Sequencing Unit, Department of Medical Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, South Africa;5. Biochemistry, Focus Area Human Metabolomics, North-West University, Potchefstroom, South Africa;6. Deltamune (Pty.) Ltd., Lyttelton, Centurion, South Africa;7. Faculdade de Medicina, Universidade Eduardo Mondlane, Maputo, Mozambique;8. Instituto Nacional de Saúde, Maputo, Mozambique |
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| Abstract: | During an ongoing diarrhea etiology surveillance in Mirzapur, Bangladesh, a rare human G6P[8] RVA strain (RVA/Human-wt/BGD/KH2288/2011/G6P[8]) was detected in a stool sample of a 7-month-old infant with acute diarrhea. Complete genotype analyses revealed that KH2288 possessed the G6-P[8]-I2-R2-C2-M2-A11-N2-T6-E2-H3 genotype constellation. Sequence analysis of the VP7 gene revealed a close phylogenetic relationship with bovine G6 strains from India, whereas, the VP4 gene segment was nearly identical to typical human P[8] strain circulating in Bangladesh and the rest of the world. Phylogenetic analysis of the remaining nine gene segments revealed a close relatedness to either animal or animal derived human RVA strain. We speculated that, strain KH2288 was a monoreassortant between a human RVA strain and a RVA strain typically infecting member of the Artiodactyla, such as cattle, goat or sheep. To our knowledge, this is the first complete genotyping report of a naturally occurring G6P[8] RVA strain, worldwide. |
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| Keywords: | Rotavirus G6P[8] Interspecies transmission Diarrhea Bangladesh |
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