和胃降逆汤防治应激性溃疡的实验研究

目的]观察和胃降逆汤对大鼠应激性溃疡的影响及可能的作用机制。方法]SD大鼠60只,随机分成模型组,和胃降逆汤低、中、高剂量组(1.25、2.5、5.0/kg),雷尼替丁组(0.05 g/kg),各组均按设计剂量灌胃给药,模型组给予等容积生理盐水,采用大鼠水束缚应激模型,观察和胃降逆汤对应激性溃疡发生、胃液分析、胃粘膜前列腺素E2(PGE2)及一氧化氮(NO)含量的影响。结果]和胃降逆汤能降低应激大鼠溃疡指数,减少胃液分泌,减少胃粘膜NO含量,增加胃粘膜PGE2含量,与模型组比较均有显著性差异(P<0.05或P<0.01),但对胃液酸度、胃蛋白酶含量无显著影响。结论]和胃降逆汤能减少胃粘膜NO含量,增加胃粘膜PGE2含量,这可能是其防治应激性溃疡的作用机制之一。

An Experimental Study on Hewei Jiangni Decoction in Preventing and Treating Stress Ulcer

Objective] To observe the effect of Hewei Jiangni Decoction (HJD) in preventing and treating stress ulcer in rats and to explore its therapeutic mechanism. Methods] Sixty SD rats were randomized into groups A (model), B (ranitidine 0.05 g/kg), C (HJD 1.25 g/kg), D (HJD 2.5 g/kg) and E (HJD 5.0 g/kg). Rat models of stress ulcer were induced by water-immersion method. Model group was given normal saline and the rats in other groups were medicated by gavage for 5 days. After treatment, effects of HJD on the incidence of stress ulcer, volume and pH value of gastric juice, prostaglandin E2 (PGE2) and nitric oxide (NO) contents in gastric mucosa were detected. Results] HJD decreased ulcer index, gastric secretion and NO content and increased the content of PGE2 ( P < 0.05 or P < 0.01 compared with the model group); however, HJD had no effect on pH value of gastric juice and pepsin activity. Conclusion] HJD has an effect in decreasing NO content and increasing PGE2 in gastric mucosa, which may be one of its preventive and therapeutic mechanisms.