| 高效液相色谱法测定普肾片中卡托普利含量 |
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| 引用本文： | 乔立业,陆崟,苏华,任海祥,王曙东. 高效液相色谱法测定普肾片中卡托普利含量[J]. 中国药业, 2013, 0(2): 20-21 |
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| 作者姓名： | 乔立业 陆崟 苏华 任海祥 王曙东 |
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| 作者单位： | 中国人民解放军南京军区总医院制剂科 |
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| 摘 要： | 目的建立测定普肾片中卡托普利含量的高效液相色谱法。方法采用C18色谱柱(250 mm×4.6 mm,5μm),0.01 mol/L磷酸二氢钠-甲醇-乙腈(75∶25∶5,磷酸调节pH至2.7～2.8)为流动相,流速为1.0 mL/min,检测波长为215 nm,柱温为40℃。结果卡托普利质量浓度在10.21～81.71μg/mL的范围内与峰面积呈良好线性关系,r=0.999 9,平均回收率为99.93%,RSD为1.25%(n=9)。结论该方法简便、准确,可作为该制剂的质量控制方法。
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| 关 键 词： | 卡托普利 高效液相色谱法 含量测定 |
Determination of Captopril in Pushen Tablets by HPLC |
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| Qiao Liye,Lu Yin,Su Hua,Ren Haixiang,Wang Shudong. Determination of Captopril in Pushen Tablets by HPLC[J]. China Pharmaceuticals, 2013, 0(2): 20-21 |
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| Authors: | Qiao Liye Lu Yin Su Hua Ren Haixiang Wang Shudong |
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| Affiliation: | (Department of Preparations,Nanjing General Hospital of Nanjing Military Region,Nanjing,Jiangsu,China 210002) |
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| Abstract: | Objective To establish a HPLC method for the determination of the captopril content in Pushen Tablets. Methods The C18 column（250 mm×4.6 mm,5 μm） was adopted with 0.01 mol/L sodium dihydrogen phosphate-methanol-acetonitrile （75：25：5, adjusting pH to 2.7-2.8 with phosphoric acid） as the mobile phase. The flow rate was 1.0 mL/min,the detection wavelength was 215 nm and the column temperature was at 40℃. Results The linear range of captopril was obtained between 10.21- 81.71μg/mL, r = 0. 999 9,the average recovery rate was 99. 93%, RSD 1.25% （n =9）. Conclusion The established method is simple,accurate,and can be used for the quality control of this preparation. |
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| Keywords: | captopril HPLC content determination |
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