Assay of dermatan sulfate cofactor (heparin cofactor II) activity in human plasma |
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Authors: | Ulrich Abildgaard Mette Lie |
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Affiliation: | Larsen Medical Department A, Aker Hospital, Oslo 5, Norway |
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Abstract: | An assay measuring the thrombin inactivating effect of human plasma in the presence of dermatan sulfate (DS) is described. Test plasma, diluted , is incubated with human thrombin in the presence of DS. Remaining thrombin is determined with chromogenic substrate 2AcOH. H-D-CHG-Ala-Arg-pNA. Three dilutions of reference plasma suffice and the standard curve is linear. Antithrombin III (AT) exerts a small (3–8%) effect in the assay.When test plasma contains heparin above 0.05 U/ml, this unspesific effect of AT increases, but it may be abolished by antibodies against AT. In a normal material (n=50), the SD of DS cofactor activity was greater (15%) than that of AT (8.7%). DS cofactor was normal in hereditary AT deficiency and in 15 patients with deep venous thrombosis. In liver cirrhosis and in DIC, both inhibitors were markedly depressed, to similar degrees (r=0.84). |
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Keywords: | Dermatan sulfate glycosaminoglycans thrombin inhibitor heparin cofactor II |
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