Studies on human protein C inhibitor in normal and factor V/VIII deficient plasmas |
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Authors: | Joyce E Gardiner John H Griffin |
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Institution: | Scripps Clinic and Research Foundation Department of Immunology 10666 North Torrey Pines Road La Jolla, CA 92037 USA |
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Abstract: | Activated protein C is a potent anticoagulant which inactivates Factors V and VIII in plasma. Normal plasma contains an inhibitor of activated protein C. A previous report from our laboratory hypothesized that the absence of this inhibitor in plasmas from patients with combined Factor V/VIII deficiency could be the molecular basis for this disease. This report demonstrates the presence of protein C inhibitor in those Factor V/VIII deficient plasmas originally studied. Freezing and thawing significantly reduced the ability of normal and Factor V/VIII deficient plasmas to inhibit activated protein C. It is suggested that this explains the conflicting literature reports describing functional assays of protein C inhibitor in Factor V/VIII deficient plasma. This observation also emphasizes that extreme care must be used in handling and storing plasma samples that are to be assayed for protein C inhibitor. |
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Keywords: | protein C inhibitor Factor V/VIII deficiency |
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