雌激素受体基因多态性与女性绝经后骨质疏松症中医辨证分型关系的研究

目的：研究中国绝经后女性雌激素受体基因多态性与绝经后骨质疏松症中医辩证分型的关系。方法：对246名中国绝经后女性（年龄44～80岁，平均65.8岁），用分子生物学的方法分析内切酶PvuⅡ、XbaI限制性长度片段多态性（RFLPs），运用双能X线骨吸收法分别测其腰椎（L1～4）和股骨（粗隆间、股骨颈、Ward′s区）骨密度，根据中医虚证辩证分型标准，将研究对象分为肾阴虚型、肾阳虚型和阴阳俱虚型，观察雌激素受体基因多态性与骨密度及中医辩证分型的关系，RFLPs用Pp(PvuⅡ)和Xx(XbaI)来表示，限制性部分缺失者用大写字母表示，存在者用小写字母表示。结果：PPxx基因型（21例）骨密度Z-score明显低于其他基因型（225例）腰椎（-0.71±0.46)g/cm^2，粗隆间（-0.31±0.58)g/cm^2，股骨颈（-0.84±0.66)g/cm^2，Ward′s区（-0.96±0.85)g/cm^2，该基因型女性中医辩证属阴阳俱虚型。结论：雌激素受体基因RFLPs与中医辩证分型有关。

Study on Relationship between Estrogen Receptor Gene Polymorphism and Syndrome Differentiation Typing of Female Postmenopausal Osteoporosis in Traditional Chinese Medicine

OBJECTIVE: To study the relationship between estrogen gene polymorphism and TCM Syndrome Differentiation of female postmenopausal osteoporosis in China. METHODS: Two hundred and forty-six Chinese postmenopausal women, age 44-80 years, mean 65.8 years, using molecular biological method to analyze the endonuclease Pvu II, Xba I restriction fragment length polymorphisms (RFLPs), with dual X-ray bone mineral density absorption meter to determine the bone mineral densities of lumbar vertebra (L1-4) and femur (intertrochanter, femur neck, Ward's region) separately. The subjects were divided into Kidney Yin deficiency type, Kidney Yang deficiency type and both Kidney Yin-Yang deficiency type, to observe the relationship between TCM and bone density as well as estrogen receptor gene polymorphism, Pp(Pvu II) and Xx(Xba I) were used to express RFLPs, the capital P and X to express the deficit of restricting sites. RESULTS: Bone mineral density of PPxx gene type (n = 21) was obviously lower than that of other gene types (n = 225), lumbar (-0.71 +/- 0.46) g/cm2, intertrochanter (-0.31 +/- 0.58) g/cm2, femur neck (-0.84 +/- 0.66) g/cm2, Ward's region (-0.96 +/- 0.85) g/cm2, the TCM Syndrome Differentiation typing of this gene type belonged to both Kidney Yin-Yang deficiency type. CONCLUSION: Estrogen receptor gene RFLPs is related to TCM Syndrome Differentiation typing.