罗格列酮对内毒素致大鼠肺组织炎症的作用机制

目的探讨过氧化物酶体增殖物激活受体γ激动剂罗格列酮对内毒素（LPS）致大鼠肺组织炎症的影响及其作用机制。方法将36只SD大鼠随机分为生理盐水对照组（A组）、罗格列酮对照组（B组）、LPS模型组（C组），以及低、中、高剂量罗格列酮组（D组、E组、F组）。HE染色观察各组肺组织炎症；检测支气管肺泡灌洗液（BALF）中的细胞总数、中性粒细胞数、巨噬细胞数；酶联免疫吸附法检测BALF中的TNF-α、IL-1β和IL-8。结果与A组比较，C组肺组织炎症积分，BALF中的细胞总数、中性粒细胞数、巨噬细胞数及TNF-α、IL-8、IL-1β均明显增加；与C组比较，D、E、F组除IL-1β外，肺组织炎症积分，BALF中的细胞总数、中性粒细胞数、巨噬细胞数及TNF-α、IL-8均明显下降，E、F组与C、D组及F组与E组比较，均有统计学差异（P均〈0．05）。结论罗格列酮能下调TNF-α和IL-8表达，减轻肺组织炎症反应。

Influence of Rosiglitazone on pulmonary inflammation induced by endotoxin in rats

Objective To investigate the influence and mechanism of Rosiglitazone ,an agonist of peroxisome proliferatots activated receptor gamma （PPAR-γ） ,on pulmonary inflammation induced by endotoxin in rats. Methods 36 male SD rats were randomly divided into 6 groups, namely saline control group（ group A ）, Rosiglitszone control group（ group B） ,pulmonary inflammation group（ group C）, low dosage group of Rosiglitazone（group D）, a moderate dosage group of Rosiglitazon （group E）, high dosage group of Rosiglitazone（ group F）. The pulmonary inflammation scoring ,cell couts, the levels of tumor necrosis factor-or （ TNF-α）, interleuldn-1β （IL-1β） and IL-8 in bronchoalveolar lavage fluid （BALF） were detected. Results Compared with group A, the pulmonary inflammation scoring, cell touts, the levels of TNF-α, IL-1β and IL-8 in BALF were significantly increased in group C （ P 〈 0.05）. Compared with group C, these inflammation index except IL-1β were gradually decreased in group D, E, F. There were statistical difference of inflammation index among group D, E, F（ P 〈 0.05 ）. Conclusion Rosiglitazone can decrease the expressions of TNF-α, IL-8, and alleviate pulmonary inflammatiouin in rats.