加压制备色谱技术结合HPLC指纹图谱导向快速分离制备红花提取物中的主要黄酮类成分（英文）

以HPLC指纹图谱为导向,应用中压反相硅胶柱色谱以及半制备高压液相色谱两种方法,对红花提取物中主要化合物进行快速分离。共分离得到了12个化学成分,包括9个黄酮醇类（1–9）和3个查尔酮类（10–12）。经波谱解析并与文献数据相比对,鉴定其结构分别为：山柰酚3-氧芸香糖苷（1）,山柰酚3-氧葡萄糖苷（2）,芦丁（3）,槲皮素3-氧葡萄糖苷（4）,6-羟基山柰酚3,6,7-三氧葡萄糖苷（5）,6-羟基山柰酚3-氧葡萄糖苷（6）,6-羟基山柰酚6,7-二氧葡萄糖苷（7）,6-羟基山柰酚3-氧芸香糖苷（8）,6-羟基山柰酚3,6-二氧葡萄糖苷7-氧葡萄糖醛酸苷（9）,异红花黄色素C（10）,红花黄色素C（11）,羟基红花黄色素A（12）。结果表明,加压制备色谱结合指纹图谱导向分离是制备靶标化合物一种快速高效的技术手段。

HPLC fingerprint-oriented preparative separation of major flavonoids from safflower extract by preparative pressurized liquid chromatography

The aim of this study was to rapidly isolate the major effective flavanoids from the extract of safflower（Carthamus tinctorius） using ODS medium pressure liquid chromatography（MPLC） and semi-preparative HPLC, guided by a developed fingerprint. Twelve compounds were isolated and their structures were elucidated as kaempferol 3-O-β-D-rutinoside（1）, kaempferol 3-O-β-D-glucoside（2）, rutin（3）, quercetin 3-O-β-D-glucoside（4）, 6-hydroxykaempferol 3,6,7-tri-O-β-D-glucoside（5）, 6-hydroxykaempferol 3-O-β-D-glucoside（6）, 6-hydroxykaempferol 6,7-di-O-β-D-glucoside（7）, 6-hydroxykaempferol 3-O-β-Drutinoside（8）, 6-hydroxykaempferol 3,6-di-O-β-D-glucosyl 7-O-β-D-glucuronide（9）, isosafflomin C（10）, safflomin C（11） and hydroxysafflor yellow A（12） by spectroscopic analysis and comparing with the literature. Our results demonstrated that preparative pressurized liquid chromatography combined with HPLC fingerprint guide is an efficient tool to isolate the target compounds quickly.