HPLC—DAD及HPLC—ESI—MS／MS法对马齿苋环多巴酰胺的定性定量研究

对中药马齿苋中环多巴酰胺类生物碱进行定性定量分析。采用HPLC—DAD及改进的HPLC-ESI-MS／MS方法，从马齿苋中鉴定了8个新环多巴酰胺Oleracein H-O和4个已知环多巴酰胺Oleracein A—D。通过分析11批样品，首次建立了马齿苋药材环多巴酰胺的HPLC指纹图谱，并同时对2个主成分Oleracein A（OA）和Oleracein B（OB）进行了定量研究，对总环多巴酰胺进行了半定量分析，结果表明，十一批马齿苋药材中OA和OB的含量分别为35．00—151．93mg／kg和40．00-150．44mg／kg，总环多巴酰胺的含量分别为314．16-928．60 mg／kg（NOA计算）或475．83—1393．00 mg／kg（NOB计算）。本实验结果为马齿苋药材的质量评价和质量控制提供了新方法。

Detection and quantification of cyclo-dopa amides in Portulaca oleracea L. by HPLC-DAD and HPLC-ESI-MS/MS

In the present study, we aimed to detect and quantify cyclo-dopa amides in Portulaca oleracea L. Together with four known ones （oleracein A-D）, we further identified eight new cyclo-dopa amides, named oleracein H-O, using an HPLC-DAD combined with an improved HPLC-ESI-MS/MS method. A standardized HPLC fingerprint of cyclo-dopa amides was generated for the first time through analyzing 11 batches ofP. oleracea. Simultaneously, the contents of two major amides, oleracein A （OA） and oleracein B （OB）, and the total amides （TID） were determined. Our results showed that the contents of OA and OB of P. oleracea ranged from 35.00 to 151.93 mg/kg and from 40.00 to 150.44 mg/kg, respectively. Moreover, TID ranged from 314.16 to 928.60 mg/kg （calculated by OA）, or from 475.83 to 1393.00 mg/kg （calculated by OB）. Taken together, our newly develooed method could be used for the aualitv assessment and oualitv control of this herb.