| 改进的抗酸染色技术在提高麻风杆菌检出率中的应用 |
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| 引用本文： | 周亚妮,;李可,;何战峰.改进的抗酸染色技术在提高麻风杆菌检出率中的应用[J].热带医学杂志,2014(10):1287-1288. |
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| 作者姓名： | 周亚妮 ;李可 ;何战峰 |
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| 作者单位： | [1]西安交通大学医学院,陕西西安710061; [2]商洛职业技术学院公共基础部,陕西商洛726000; [3]商洛疗养院,陕西商洛726000 |
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| 基金项目： | 陕西省教育科学规划课题（SGH13557） |
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| 摘 要： | 目的将传统的萋-尼(Ziehl-Neelsen)染色法进行改进,提高麻风杆菌的阳性检出率。方法对商洛疗养院68例麻风病人和121例疑似病例用传统的萋-尼(Ziehl-Neelsen)染色法和改进的萋-尼(Ziehl-Neelsen)染色法进行实验室检测,病理切片做进一步比较。结果改进的抗酸染色法麻风杆菌阳性检出率明显高于传统的萋-尼(Ziehl-Neelsen)染色法,前者阳性检出率为70.4%,后者为44.4%,差异有统计学意义(χ2=7.07,P0.05)。改进的抗酸染色法麻风杆菌切片着色率高、染色效果佳。结论改进的抗酸染色法优于传统的抗酸染色法,为麻风病的实验室诊断和筛查提供了靶方向。
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| 关 键 词： | 抗酸染色法 麻风杆菌 检出率 |
Improved acid-fast staining techniques to improve the detection rate of Mycobacterium leprae in the application |
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| Institution: | ZHOU Ya-ni,LI Ke,HE Zhan-feng (1.School of Medicine, Xi＇ an Jiaotong University, Xi＇ an, Shaanxi 710061 ; 2.Department of Public Infrastructure, Shangluo Vocational and Technical College, Shangluo, Shaanxi 726000; 3.Shangluo Sanatorium, Shangluo, Shaanxi 726000, China) |
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| Abstract: | Objective The traditional luxuriant to improve Nigeria（Ziehl-Neelsen） staining, increased the positive rate of leprosy. Methods Use leprosy 68 patients and 121 suspected case from Shangluo sanatorium in laboratory testing about Decolorization of raditional Ziehl- Neelsen acid- fast staining and improved study of acid- fast staining techniques.Results Improved detection of acid-fast staining in the positive rate of leprosy patients is higher than the traditional aspects of Ziehl-Neelsen acid-fast staining,the former positive rate of 70.4%, the later is 44.4%;improved acid-fast staining of mycobacterium leprae slicecoloring rate. Conclusion The improved acid-fast staining method is superior to conventional acid-fast staining of leprosy for the laboratory diagnosis of leprosy and provides targets creening direction. |
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| Keywords: | acid-fast staining Mycobacterium leprae detection rate |
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